Overview

  • Product nameAnti-Troponin T1 antibody [MAC 145]
    See all Troponin T1 primary antibodies
  • Description
    Rat monoclonal [MAC 145] to Troponin T1
  • SpecificityAntibody reacts with troponin-T in Lethocerus, Drosophila and dragonfly muscles (flight and non-flight).
  • Tested applicationsSuitable for: ICC/IF, Electron Microscopy, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster, Dragon Fly, Lethocerus indicus (Waterbug)
    Does not react with: Caenorhabditis elegans
  • Immunogen

    Troponin-tropomyosin complex purified from Lethocerus indicus (Waterbug)flight muscle

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberMAC 145
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Electron Microscopy
WB
  • Application notesElectron Microscopy: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTroponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • Involvement in diseaseNemaline myopathy 5
    • Sequence similaritiesBelongs to the troponin T family.
    • Information by UniProt
    • Alternative names
      • ANM antibody
      • MGC104241 antibody
      • NEM5 antibody
      • Skeletal antibody
      • Slow antibody
      • slow skeletal muscle antibody
      • Slow skeletal muscle troponin T antibody
      • sTnT antibody
      • Tnnt1 antibody
      • TNNT1_HUMAN antibody
      • TNT antibody
      • TnTs antibody
      • Troponin T antibody
      • Troponin T slow skeletal muscle antibody
      • Troponin T type 1 (skeletal slow) antibody
      • troponin T1 skeletal slow antibody
      see all

    References for Anti-Troponin T1 antibody [MAC 145] (ab50576)

    This product has been referenced in:
    • Agianian B  et al. A troponin switch that regulates muscle contraction by stretch instead of calcium. EMBO J 23:772-9 (2004). Read more (PubMed: 14765112) »
    • Fitzhugh G & Marden J Maturational changes in troponin T expression, Ca2+-sensitivity and twitch contraction kinetics in dragonfly flight muscle J Exp Biol 200:1473-82 (1997). Read more (PubMed: 9319373) »

    See all 4 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Crab Cell (Neuron)
    Specification Neuron
    Permeabilization Yes - Tween
    Fixative Formaldehyde
    Username

    Prof. Martin Mendelson

    Verified customer

    Submitted Oct 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"