Overview

  • Product nameAnti-TROY antibody
    See all TROY primary antibodies
  • Description
    Rabbit polyclonal to TROY
  • Specificityab12126 reacts with TROY.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 29-44, 204-219, and 409-421 of Human TROY.

  • Positive control
    • T98G cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab12126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 50.8 kDa). For optimal results, primary antibody incubations should be performed at room temperature.

Target

  • FunctionCan mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death.
  • Tissue specificityHighly expressed in prostate. Detected at lower levels in thymus, spleen, testis, uterus, small intestine, colon and peripheral blood leukocytes.
  • Sequence similaritiesContains 3 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL023044 antibody
    • AW123854 antibody
    • TAJ alpha antibody
    • TAJ antibody
    • TAJ-alpha antibody
    • TNFRSF19 antibody
    • TNFRSF19 tumor necrosis factor receptor superfamily, member 19 antibody
    • TNR19_HUMAN antibody
    • Toxicity and JNK inducer antibody
    • TRADE antibody
    • TROY antibody
    • Tumor necrosis factor receptor superfamily member 19 antibody
    • UNQ1888/PRO4333 antibody
    see all

Anti-TROY antibody images



  • Predicted band size : 50.8 kDa


    TROY detection by Western blot. The analysis of human TROY using this antibody in T98G, a human glioblastoma cell line extract. A protein band of approximately 46 kDa should be detected.
  • ICC/IF image of ab12126 stained Mouse embryonic stem cells cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TROY antibody (ab12126)

This product has been referenced in:
  • Fernandez-Enright F  et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014). WB ; Human . Read more (PubMed: 24448210) »
  • Jacobs VL  et al. Propentofylline targets TROY, a novel microglial signaling pathway. PLoS One 7:e37955 (2012). WB ; Rat . Read more (PubMed: 22649568) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"