Recombinant
RabMAb

Anti-TROY antibody [EPR3213(2)] (ab138502)

Overview

  • Product name
    Anti-TROY antibody [EPR3213(2)]
    See all TROY primary antibodies
  • Description
    Rabbit monoclonal [EPR3213(2)] to TROY
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human prostate cancer, A431, LnCaP, PC3, U87-MG, and fetal brain lysates, Human prostate tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab138502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Can mediate activation of JNK and NF-kappa-B. May promote caspase-independent cell death.
    • Tissue specificity
      Highly expressed in prostate. Detected at lower levels in thymus, spleen, testis, uterus, small intestine, colon and peripheral blood leukocytes.
    • Sequence similarities
      Contains 3 TNFR-Cys repeats.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AL023044 antibody
      • AW123854 antibody
      • TAJ alpha antibody
      • TAJ antibody
      • TAJ-alpha antibody
      • TNFRSF19 antibody
      • TNFRSF19 tumor necrosis factor receptor superfamily, member 19 antibody
      • TNR19_HUMAN antibody
      • Toxicity and JNK inducer antibody
      • TRADE antibody
      • TROY antibody
      • Tumor necrosis factor receptor superfamily member 19 antibody
      • UNQ1888/PRO4333 antibody
      see all

    Images

    • Overlay histogram showing PC3 cells stained with ab138502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab138502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-TROY antibody [EPR3213(2)] (ab138502) at 1/1000 dilution

      Lane 1 : Human prostate cancer lysate
      Lane 2 : A431 lysate
      Lane 3 : LnCaP lysate
      Lane 4 : PC3 lysate
      Lane 5 : U87-MG lysate
      Lane 6 : fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
    • Immunohistochemical analysis of paraffin embedded Human prostate tissue labelling TROY with ab138502 antibody at a dilution of 1/50.

    References

    ab138502 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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