Anti-TRP1 antibody (ab83774)


  • Product nameAnti-TRP1 antibody
    See all TRP1 primary antibodies
  • Description
    Rabbit polyclonal to TRP1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 396-445 (


    ) of Human TRP1 (NP_000541).

  • Positive control
    • 721_B cell lysate



Our Abpromise guarantee covers the use of ab83774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionOxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
  • Tissue specificityPigment cells.
  • PathwayPigment biosynthesis; melanin biosynthesis.
  • Involvement in diseaseDefects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
  • Sequence similaritiesBelongs to the tyrosinase family.
  • Cellular localizationMelanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 antibody
    • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
    • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
    • b-PROTEIN antibody
    • CAS2 antibody
    • Catalase B antibody
    • CATB antibody
    • DHICA oxidase antibody
    • Glycoprotein 75 antibody
    • GP75 antibody
    • Melanoma antigen gp75 antibody
    • OCA3 antibody
    • TRP antibody
    • TRP-1 antibody
    • TRP1 antibody
    • Tyrosinase related protein 1 antibody
    • Tyrosinase-related protein 1 antibody
    • TYRP antibody
    • TYRP1 antibody
    • TYRP1_HUMAN antibody
    • TYRRP antibody
    see all

Anti-TRP1 antibody images

  • Anti-TRP1 antibody (ab83774) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • ab83774 staining TRP1 in human melanoma MNT-1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 1% BSA for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution.

    See Abreview

  • ab83774 at 5 µg/ml staining TRP1 in Human skin by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-TRP1 antibody (ab83774)

ab83774 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (epidermis)
Specification epidermis
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

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Verified customer

Submitted May 29 2013

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (melanoma (oral))
Specification melanoma (oral)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375 melanoma cell line)
Loading amount 10000 cells
Specification A375 melanoma cell line
Gel Running Conditions Reduced Denaturing (10% acrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted May 31 2012

It was nice speaking to you on the phone too!

I am very happy to hear thay you would like to take up my offer of testing ab104864 and ab83774 with your human and mouse samples respectively. I have generated the testing discount codes as below...

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Thank you for contacting us in regards to antibodies ab104864, ab83774 and ab52493.

As discussed over the phone, the western blot presented on the datasheet of Anti-Hyaluronan synthase 1 antibody [3E10] (ab104864) was performed using the reco...

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Thank you very much for your interest in ab83774. To our knowledge, ab83774 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab83774 now, test it in mouse and submit feedback to us in the form of an A...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human melanoma line MNT-1)
Loading amount 20 µg
Specification human melanoma line MNT-1
Treatment control (C) or WIPI1 (W) siRNA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Ms. Hsiang Ho

Verified customer

Submitted May 05 2011