Anti-TRP1 antibody [TA99] (ab3312)
Key features and details
- Mouse monoclonal [TA99] to TRP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TRP1 antibody [TA99]
See all TRP1 primary antibodies -
Description
Mouse monoclonal [TA99] to TRP1 -
Host species
Mouse -
Specificity
ab3312 is specific for melanomas and does not react with carcinomas and sarcomas. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
SK-MEL-23 Melanoma cell line.
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Positive control
- In Western Blot, this antibody gave a positive signal in human skin tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TA99 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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Notes |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
Target
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Function
Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. -
Tissue specificity
Pigment cells. -
Pathway
Pigment biosynthesis; melanin biosynthesis. -
Involvement in disease
Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. -
Sequence similarities
Belongs to the tyrosinase family. -
Cellular localization
Melanosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 7306 Human
- Omim: 115501 Human
- SwissProt: P17643 Human
- Unigene: 270279 Human
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Alternative names
- 5 antibody
- 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibody
see all
Images
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Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab3312 has been referenced in 31 publications.
- Wei X et al. Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF. Exp Dermatol 31:1253-1263 (2022). PubMed: 35514241
- Netcharoensirisuk P et al. Cajanin Suppresses Melanin Synthesis through Modulating MITF in Human Melanin-Producing Cells. Molecules 26:N/A (2021). PubMed: 34641584
- Aktary Z et al. A role for Dynlt3 in melanosome movement, distribution, acidity and transfer. Commun Biol 4:423 (2021). PubMed: 33772156
- Guo D et al. RAB27A/Melanophilin Blocker Inhibits Melanoma Cell Motility and Invasion. J Invest Dermatol 140:1470-1473.e3 (2020). PubMed: 31978414
- Li B et al. Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes. Acta Pharm 70:539-549 (2020). PubMed: 32412435