Overview

  • Product name
    Anti-TRP1 antibody [TA99]
    See all TRP1 primary antibodies
  • Description
    Mouse monoclonal [TA99] to TRP1
  • Host species
    Mouse
  • Specificity
    ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    SK-MEL-23 Melanoma cell line.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in human skin tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19841138

Target

  • Function
    Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
  • Tissue specificity
    Pigment cells.
  • Pathway
    Pigment biosynthesis; melanin biosynthesis.
  • Involvement in disease
    Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
  • Sequence similarities
    Belongs to the tyrosinase family.
  • Cellular localization
    Melanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 antibody
    • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
    • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
    • b-PROTEIN antibody
    • CAS2 antibody
    • Catalase B antibody
    • CATB antibody
    • DHICA oxidase antibody
    • Glycoprotein 75 antibody
    • GP75 antibody
    • Melanoma antigen gp75 antibody
    • OCA3 antibody
    • TRP antibody
    • TRP-1 antibody
    • TRP1 antibody
    • Tyrosinase related protein 1 antibody
    • Tyrosinase-related protein 1 antibody
    • TYRP antibody
    • TYRP1 antibody
    • TYRP1_HUMAN antibody
    • TYRRP antibody
    see all

Images

  • Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 72 kDa
    Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • ab3312 staining TRP1 in B16 mouse melanoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% goat serum for 1 hour at 22°C. The primary antibody was diluted 1/200 in 5% goat serum/1% BSA in PBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Mouse polyclonal, diluted 1/500.

    See Abreview

  • Paraformaldehyde-fixed, 0.1% tritonx-100 - permeabilized human skin tissue stained for TRP1 (Red) using ab3312 at 1/300 dilution in immunohistochemical analysis. Phalloidin (Green) DAPI (Blue).

    See Abreview

References

This product has been referenced in:
  • Zhou D  et al. A novel P53/POMC/Gas/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation. J Cell Mol Med 21:802-815 (2017). WB, IHC-P ; Human . Read more (PubMed: 27885802) »
  • Liao S  et al. Effects of two chronic stresses on mental state and hair follicle melanogenesis in mice. Exp Dermatol N/A:N/A (2017). IHC ; Mouse . Read more (PubMed: 28480983) »

See all 12 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abreviews
Application
ELISA
Sample
Human Serum (melanoma patient serum)
Specification
melanoma patient serum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Type
Direct
Username

Frau. Mirjam Fässler

Verified customer

Submitted Sep 12 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
0.1% triton, 2.5% goat serum, 2% BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Tissue sections (Human Skin)
Specification
Human Skin
Permeabilization
Yes - 0.1% tritonx-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 09 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SK-MEL-28)
Specification
SK-MEL-28
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X in PBS
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16 mouse melanoma cell line)
Specification
B16 mouse melanoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. James Harris

Verified customer

Submitted Sep 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up