The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
Use at an assay dependent concentration. PubMed: 19841138
Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
Pigment biosynthesis; melanin biosynthesis.
Involvement in disease
Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa Observed band size: 72 kDa Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [TA99] (ab3312)This image is courtesy of an Abreview submitted by James Harris.
ab3312 staining TRP1 in B16 mouse melanoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% goat serum for 1 hour at 22°C. The primary antibody was diluted 1/200 in 5% goat serum/1% BSA in PBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Mouse polyclonal, diluted 1/500.
Zhou D et al. A novel P53/POMC/Gas/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation. J Cell Mol Med21:802-815 (2017).
Read more (PubMed: 27885802) »
Liao S et al. Effects of two chronic stresses on mental state and hair follicle melanogenesis in mice. Exp DermatolN/A:N/A (2017).
Read more (PubMed: 28480983) »