Anti-TRP2 antibody (ab103463)

Overview

  • Product nameAnti-TRP2 antibody
    See all TRP2 primary antibodies
  • Description
    Rabbit polyclonal to TRP2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 68-98 of Human TRP2.

  • Positive control
    • Human skin

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab103463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in regulating eumelanin and phaeomelanin levels.
  • PathwayPigment biosynthesis; melanin biosynthesis.
  • Sequence similaritiesBelongs to the tyrosinase family.
  • Cellular localizationMelanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

Anti-TRP2 antibody images

  • Anti-TRP2 antibody (ab103463) at 1/100 dilution + A375 cell lysate at 35 µg

    Predicted band size : 59 kDa
  • ab103463, at a 1/50 dilution, staining TRP2 in formalin-fixed, paraffin-embedded breast carcinoma, using a peroxidase conjugated secondary antibody and DAB staining.
  • ab103643 staining TRP2 in Human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Incubation with the primary antibody was followed by preoxidase conjugation of the secondary antibody and DAB staining. TRP2 staining is indicated by arrows.

References for Anti-TRP2 antibody (ab103463)

ab103463 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (1 kidney, 2 liver, 3 heart, 4 positive control)
Specification 1 kidney, 2 liver, 3 heart, 4 positive control
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 13 2014

Thank you for your phone call. I have created the following discount codes for you:


DISCOUNT CODE: xxx for ab83774
DISCOUNT CODE: xxx for ab103463
DISCOUNT CODE: xxx for ab46545

Expiration date: xxx

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (oral melanoma)
Specification oral melanoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375 - melanoma cell line)
Loading amount 10000 cells
Specification A375 - melanoma cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 31 2012

Thank you for contacting us. I found the dog sequences and the immunogen forantibody ab103463 has 88% homology with dog, so it is likely to work. That would be the antibody I suggest you try for TRP1. As far as TRP1, I would recommend ab83774. It has n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"