Overview

  • Product name
  • Description
    Rabbit polyclonal to TRP2
  • Specificity
    ab74073 detects endogenous levels of total TRP2 protein.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRP2 (internal sequence). synthesized peptide derived from internal of human TRP2.

  • Positive control
    • WB: Extracts from K562 cells ICC/IF: A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.

TRP2 protein is a single-pass type I membrane protein, these types of proteins known to aggregate when boiled therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70C instead of boiling.   

ELISA 1/40000.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Involved in regulating eumelanin and phaeomelanin levels.
  • Pathway
    Pigment biosynthesis; melanin biosynthesis.
  • Sequence similarities
    Belongs to the tyrosinase family.
  • Cellular localization
    Melanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

Anti-TRP2 antibody images

  • ab74043 staining TRP2 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using a TRIS/EDTA buffer pH 9.0. Samples were then permeabilized, followed by incubation with the primary antibody at a 1/1000 dilution for 30 minutes at 20°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
  • ab74073 staining TRP2 in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-TRP2 antibody (ab74073) at 1/500 dilution

    Lane 1 : Extracts from K562 cells with no peptide
    Lane 2 : Extracts from K562 cells with peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 117 kDa,39 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of TRP2 in A549 cells, using ab74073 at a dilution of 1/500.
    Left hand panel: no peptide
    right hand panel: with immunising peptide.

References for Anti-TRP2 antibody (ab74073)

This product has been referenced in:
  • Liao S  et al. Effects of two chronic stresses on mental state and hair follicle melanogenesis in mice. Exp Dermatol N/A:N/A (2017). IHC ; Mouse . Read more (PubMed: 28480983) »
  • Coelho PA  et al. Over-expression of Plk4 induces centrosome amplification, loss of primary cilia and associated tissue hyperplasia in the mouse. Open Biol 5:N/A (2015). Read more (PubMed: 26701933) »

See all 11 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Ahmar Aziz

Verified customer

Submitted Feb 10 2016

The K562 cells were not transfected. I am not sure how they were chosen for the western blot test, as I have not found any papers that used K562 to study Trp2. Most of the studies are using melanocytes, for instance the paper at this link: http://www.n...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (melan-ink melanocytes)
Specification
melan-ink melanocytes
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Mr. Heiko Locher

Verified customer

Submitted Sep 23 2013

Thank you very much for your interest our AbTrial program.

To our knowledge, ab74073 has not been tested in FACS. However by participating in our AbTrial program you can now use our products in an untested application or species without financ...

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Thank you for contacting Abcam regarding ab74073.


Regarding the specificity of this antibody, it will recognize both endogenous TRP2 as well as exogenous protein (cells transfected with full length TRP2). Please see the customer Abrevie...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100
Blocking step
3% BSA, 5% NGS, 0.2%TritonX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 09 2012


I am very pleased to hear you would like to accept our offer and test ab74073 in IHC-Fr]. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC-Fr and include this code in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (B16 mouse melanoma cell line)
Specification
B16 mouse melanoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. James Harris

Verified customer

Submitted Sep 27 2011

Thank you for your inquiry. Unfortunately, we do not know whether the light chains in these antibodies (ab3312 and ab74073) are lambda (λ) or kappa (κ). This has not been tested. If you require a secondary antibody for these primary a...

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Thank you once again for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investig...

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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