Overview

  • Product nameAnti-TRPA1 antibody
    See all TRPA1 primary antibodies
  • Description
    Mouse monoclonal to TRPA1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: IPNADKSLEM EILKQKYRLK DLTFLLEKQH ELIKLIIQKM EIISETEDDD SHCSFQDRFK KEQMEQRNSR WNTVLRAVKA KTHHL, corresponding to amino acids 1033-1118 of Human TRPA1

Properties

Applications

Our Abpromise guarantee covers the use of ab56458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
    • Tissue specificityExpressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
    • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family.
      Contains 15 ANK repeats.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANKTM 1 antibody
      • ANKTM1 antibody
      • Ankyrin-like with transmembrane domains protein 1 antibody
      • Transformation sensitive protein p120 antibody
      • Transformation-sensitive protein p120 antibody
      • Transient receptor potential cation channel subfamily A member 1 antibody
      • TRPA 1 antibody
      • Trpa1 antibody
      • TRPA1_HUMAN antibody
      see all

    Anti-TRPA1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56458 TRPA1 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa

    References for Anti-TRPA1 antibody (ab56458)

    ab56458 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Dorsal root ganglia, all spinal levels)
    Loading amount 30 µg
    Specification Dorsal root ganglia, all spinal levels
    Gel Running Conditions Reduced Denaturing (8% acrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Andrew Grant

    Verified customer

    Submitted Nov 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"