Overview

  • Product name
  • Description
    Mouse monoclonal to TRPA1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: IPNADKSLEM EILKQKYRLK DLTFLLEKQH ELIKLIIQKM EIISETEDDD SHCSFQDRFK KEQMEQRNSR WNTVLRAVKA KTHHL, corresponding to amino acids 1033-1118 of Human TRPA1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
    • Tissue specificity
      Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
    • Sequence similarities
      Belongs to the transient receptor (TC 1.A.4) family.
      Contains 15 ANK repeats.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANKTM 1 antibody
      • ANKTM1 antibody
      • Ankyrin-like with transmembrane domains protein 1 antibody
      • Transformation sensitive protein p120 antibody
      • Transformation-sensitive protein p120 antibody
      • Transient receptor potential cation channel subfamily A member 1 antibody
      • TRPA 1 antibody
      • Trpa1 antibody
      • TRPA1_HUMAN antibody
      see all

    Images

    • Western blot against tagged recombinant protein immunogen using ab56458 TRPA1 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa

    References

    ab56458 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Dorsal root ganglia, all spinal levels)
    Loading amount
    30 µg
    Specification
    Dorsal root ganglia, all spinal levels
    Gel Running Conditions
    Reduced Denaturing (8% acrylamide)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Andrew Grant

    Verified customer

    Submitted Nov 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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