Overview

  • Product name
  • Description
    Rabbit polyclonal to TRPC3
  • Specificity
    ab51560 can be used for the detection of TRPC3.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to 14 amino acids near to the N terminal of Human TRPC3

  • Positive control
    • Mouse heart tissue and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab51560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).Can be blocked with TRPC3 peptide (ab75651).

Target

  • Relevance
    TRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.
  • Cellular localization
    Cell Membrane; multi-pass membrane protein
  • Database links
  • Alternative names
    • Htrp 3 antibody
    • Htrp3 antibody
    • MGC124333 antibody
    • Mtrp3 antibody
    • Receptor activated cation channel TRP3 antibody
    • Short transient receptor potential channel 3 antibody
    • Transient receptor potential cation channel subfamily C member 3 antibody
    • transient receptor potential cation channel subfamily C member 3 variant c antibody
    • Transient receptor potential channel 3 antibody
    • TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody
    • Transient receptor protein 3 antibody
    • Trp related protein 3 antibody
    • TRP3 antibody
    • TrpC3 antibody
    • Trrp3 antibody
    see all

Images

  • Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody ab51560 at 10 µg/ml.

References

This product has been referenced in:
  • Hofhuis J  et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). Read more (PubMed: 28104817) »
  • Nakipova OV  et al. Store-operated Ca2+ entry supports contractile function in hearts of hibernators. PLoS One 12:e0177469 (2017). Read more (PubMed: 28531217) »

See all 7 Publications for this product

Customer reviews and Q&As

The exact sequence is proprietary information, but we would be happy to run an alignment and forward the results.


Please let us know if you have any more questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: dako low pH
Permeabilization
No
Blocking step
No blocking step used for 30 minute(s) · Concentration: 2%
Username

Dr. Mark Southwood

Verified customer

Submitted Oct 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (platelets)
Loading amount
20 µg
Specification
platelets
Treatment
Thrombin 0.1U/ml for 3 min
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Dr Juan A. Rosado

Verified customer

Submitted Apr 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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