Overview

  • Product nameAnti-TRPC3 antibody
    See all TRPC3 primary antibodies
  • Description
    Rabbit polyclonal to TRPC3
  • Specificityab51560 can be used for the detection of TRPC3.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to 14 amino acids near to the N terminal of Human TRPC3

  • Positive control
    • Mouse heart tissue and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab51560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).Can be blocked with TRPC3 peptide (ab75651).

Target

  • RelevanceTRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.
  • Cellular localizationCell Membrane; multi-pass membrane protein
  • Database links
  • Alternative names
    • Htrp 3 antibody
    • Htrp3 antibody
    • MGC124333 antibody
    • Mtrp3 antibody
    • Receptor activated cation channel TRP3 antibody
    • Short transient receptor potential channel 3 antibody
    • Transient receptor potential cation channel subfamily C member 3 antibody
    • transient receptor potential cation channel subfamily C member 3 variant c antibody
    • Transient receptor potential channel 3 antibody
    • TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody
    • Transient receptor protein 3 antibody
    • Trp related protein 3 antibody
    • TRP3 antibody
    • TrpC3 antibody
    • Trrp3 antibody
    see all

Anti-TRPC3 antibody images

  • Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody ab51560 at 10 µg/ml.

References for Anti-TRPC3 antibody (ab51560)

This product has been referenced in:
  • Letavernier E  et al. Williams-Beuren syndrome hypercalcemia: is TRPC3 a novel mediator in calcium homeostasis? Pediatrics 129:e1626-30 (2012). ICC/IF, IHC ; Human . Read more (PubMed: 22566418) »
  • Woodard GE  et al. TRPC3 regulates agonist-stimulated Ca2+ mobilization by mediating the interaction between type I inositol 1,4,5-trisphosphate receptor, RACK1, and Orai1. J Biol Chem 285:8045-53 (2010). WB ; Human . Read more (PubMed: 20022948) »

See all 4 Publications for this product

Product Wall

The exact sequence is proprietary information, but we would be happy to run an alignment and forward the results.


Please let us know if you have any more questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: dako low pH
Permeabilization No
Blocking step No blocking step used for 30 minute(s) · Concentration: 2%
Username

Dr. mark southwood

Verified customer

Submitted Oct 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelets)
Loading amount 20 µg
Specification platelets
Treatment Thrombin 0.1U/ml for 3 min
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Dr Juan A. Rosado

Verified customer

Submitted Apr 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"