Overview

  • Product nameAnti-TRPC4 antibody
    See all TRPC4 primary antibodies
  • Description
    Rabbit polyclonal to TRPC4
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within residues: CPFKSEKVVV EDTVPIIPKE KHAKEEDSSI DYDLNLPDTV THEDYVTTRL , corresponding to internal sequence amino acids 928-977 of Human TRPC4 (NP_057263).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionForm a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion.
  • Tissue specificityStrongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
    Contains 4 ANK repeats.
  • DomainThe protein 4.1-binding domain (654-685) is required for binding to EPB41L2 and channel activation.
    The calmodulin- and inositol 1,4,5-trisphosphate receptor-binding (CIRB) domain (695-724) is sufficient for the interaction with SESTD1.
    The spectrin-binding domain (730-758) is required for binding to SPTAN1 and SPTBN5.
  • Post-translational
    modifications
    Phosphorylation modulates TRPC channel function by regulating the level of TRPC4 at the cell surface and by increasing the association with SLC9A3R1/NHERF.
  • Cellular localizationMembrane. Cell membrane. Enhanced insertion into the cell membrane after activation of the EGF receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCE1 antibody
    • HTRP 4 antibody
    • hTrp-4 antibody
    • hTrp4 antibody
    • MGC119570 antibody
    • MGC119571 antibody
    • MGC119572 antibody
    • MGC119573 antibody
    • RP11-10M8.1 antibody
    • Short transient receptor potential channel 4 antibody
    • STRPC4 antibody
    • Transient receptor potential 4 antibody
    • transient receptor potential cation channel subfamily C antibody
    • Transient receptor potential cation channel subfamily C member 4 antibody
    • transient receptor potential channel 4 antibody
    • TRP 4 antibody
    • trp related protein 4 antibody
    • Trp-related protein 4 antibody
    • TRP4 antibody
    • TRPC 4 antibody
    • TrpC4 antibody
    • TRPC4 protein antibody
    • TRPC4_HUMAN antibody
    see all

Anti-TRPC4 antibody images

  • Anti-TRPC4 antibody (ab83689) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling TRPC4 with ab83689 at 5µg/ml.



  • Predicted band size : 112 kDa
    Western Blot using ab83689 at 1.0ug/ml. Sample type: Human 293T cell Lysate


  • Predicted band size : 112 kDa
    Western Blot using ab83689 at 1.0ug/ml. Sample type: Human HepG2 cell Lysate


  • Predicted band size : 112 kDa
    Western Blot using ab83689 at 1.0ug/ml. Sample type: Human MCF7 cell Lysate

References for Anti-TRPC4 antibody (ab83689)

ab83689 has not yet been referenced specifically in any publications.

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