Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)
Key features and details
- Mouse monoclonal [3F2.H10.F2] to TRPC6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-TRPC6 antibody [3F2.H10.F2]
See all TRPC6 primary antibodies -
Description
Mouse monoclonal [3F2.H10.F2] to TRPC6 -
Host species
Mouse -
Specificity
Cross-reactivity with TRPC6 from other sources has not been determined. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide corresponding to a region near the C terminal of Human TRPC6 (NP_004612).
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Positive control
- Human adrenal (cortex) tissue
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from concentrated tissue culture supernate by Protein A chromatography. -
Clonality
Monoclonal -
Clone number
3F2.H10.F2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 106 kDa.
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 106 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. -
Tissue specificity
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. -
Involvement in disease
Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
Contains 4 ANK repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7225 Human
- Entrez Gene: 22068 Mouse
- Omim: 603652 Human
- SwissProt: Q9Y210 Human
- SwissProt: Q61143 Mouse
- Unigene: 159003 Human
- Unigene: 325086 Mouse
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Alternative names
- bZ1P14.9 antibody
- FLJ11098 antibody
- FLJ14863 antibody
see all
Images
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ab105845 at 2.5 µg/ml staining TRPC6 in Human adrenal (cortex) tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows strong staining with minimal background staining. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. -
Western blot analysis of ab105845 with mouse kidney (10ug) at the dilution 1/1000.Secondary antibody (DyLight™ 649 donkey anti-mouse) was used at the dilution 1/20000
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab105845 has not yet been referenced specifically in any publications.