Overview

  • Product nameAnti-TRPC6 antibody
    See all TRPC6 primary antibodies
  • Description
    Rabbit polyclonal to TRPC6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Horse, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse TRPC6.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse lung; Mouse kidney; Rat brain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 107 kDa).

Target

  • FunctionThought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificityExpressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in diseaseDefects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all

Anti-TRPC6 antibody images

  • All lanes : Anti-TRPC6 antibody (ab101622) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 107 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-TRPC6 antibody (ab101622)

ab101622 has not yet been referenced specifically in any publications.

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