Our Abpromise guarantee covers the use of ab62461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).Can be blocked with TRPC6 peptide (ab106953).|
K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 (red) using ab62461 (5 µg/ml) in ICC/IF.
Paraffin embedded mouse lung tissue stained for TRPC6 with ab62461 (10 µg/ml) in immunohistochemical analysis.
K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 using ab62461 (2 µg/ml) in ICC.