Overview

  • Product nameAnti-TRPC6 antibody
    See all TRPC6 primary antibodies
  • Description
    Rabbit polyclonal to TRPC6
  • SpecificityThis antibody does potentially cross-react with TRPC3.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPC6 (N terminal).
    Database link: Q9Y210
    (Peptide available as ab106953)

  • Positive control
    • K562 cell lysate and mouse lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).Can be blocked with TRPC6 peptide (ab106953).

Target

  • FunctionThought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificityExpressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in diseaseDefects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all

Anti-TRPC6 antibody images

  • Lane 1 : Anti-TRPC6 antibody (ab62461) at 0.5 µg/ml
    Lane 2 : Anti-TRPC6 antibody (ab62461) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 106 kDa
    Observed band size : 106 kDa
  • Immunohistochemistry of TRPC6 in paraffin embedded mouse lung tissue section with ab62461 at 10 µg/ml.
  • Immunocytochemistry of TRPC6 in K562 cells section with ab62461 at 5 ug/ml.

  • Immunocytochemistry of TRPC6 in K562 cells section with ab62461 at 2 ug/ml.

References for Anti-TRPC6 antibody (ab62461)

This product has been referenced in:
  • Takada H  et al. Mechanosensitive ATP release from hemichannels and Ca²? influx through TRPC6 accelerate wound closure in keratinocytes. J Cell Sci 127:4159-71 (2014). ICC ; Human . Read more (PubMed: 25097230) »
  • Bernaldo de Quirós S  et al. Identification of TRPC6 as a possible candidate target gene within an amplicon at 11q21-q22.2 for migratory capacity in head and neck squamous cell carcinomas. BMC Cancer 13:116 (2013). WB ; Human . Read more (PubMed: 23497198) »

See all 2 Publications for this product

Product Wall

I was not able to obtain the exact sequence of the immunogen, as it is considered proprietary by our source for this antibody. I did learn that the immunogen shares 92% identity with rat TRPC6. There are 13 contiguous identical amino acids out of 14. <...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Mouse Cell (Podocyte)
Specification Podocyte
Permeabilization Yes - 0.1% triton-X
Fixative Formaldehyde
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Submitted Jul 27 2012

Please find a new discount code that you can use to submit your Abreview for ab62461 in IF:

new discount code - 1*00%ABR-TBVX1, exp*



If there is anything else I can help you with, please let me know.

Thank you for contacting us. After consulting with the laboratory regarding ab62461, I have discovered that though the rat and mouse sequence are quite similar, they did test in rat and did not receive a positive signal.

I hope this informatio...

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Thank you very much for your interest in our anti-TRPC6 antibodies.

While we do offer six products which work in human tissues, to our knowledge, none of these products have been tested in FACS on human cells. Therefore, I can offer a discoun...

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I am very pleased to hear you would like to accept our offer and test ab62461 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"