The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Transient receptor potential cation channel, subfamily M, member 3 (TRPM3) belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation selective channels important for cellular calcium signaling and homeostasis. TRPM3 mediates calcium entry, and this entry is potentiated by calcium store depletion. Its activity is increased by reduction in extracellular osmolarity, by store depletion and muscarinic receptor activation. The TRPM3-mediated calcium entry is inhibited by the nonselective calcium channel blocker lanthanide gadolinium. Expressed primarily in the kidney and, at lower levels, in brain, testis, ovary, pancreas and spinal cord. In the kidney, expressed predominantly in the collecting tubular epithelium in the medulla, medullary rays, and periglomerular regions; in the brain, highest levels are found in the cerebellum, choroid plexus, the locus coeruleus, the posterior thalamus and the substantia nigra. Down-regulated in renal tumors compared to normal kidney. Alternatively spliced transcript variants encoding different isoforms have been identified.