Overview

  • Product nameAnti-TRPM3 antibody
    See all TRPM3 primary antibodies
  • Description
    Rabbit polyclonal to TRPM3
  • Specificityab32993 recognises Transient receptor potential cation channel subfamily M member 3 (TRPM3).
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: YLRDTPPVPV VVCDGSGRAS DILAFGHKYS EEGGLINESL RDQLLVTIQK, corresponding to internal sequence amino acids 197-246 of Human TRPM3

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceTransient receptor potential cation channel, subfamily M, member 3 (TRPM3) belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation selective channels important for cellular calcium signaling and homeostasis. TRPM3 mediates calcium entry, and this entry is potentiated by calcium store depletion. Its activity is increased by reduction in extracellular osmolarity, by store depletion and muscarinic receptor activation. The TRPM3-mediated calcium entry is inhibited by the nonselective calcium channel blocker lanthanide gadolinium. Expressed primarily in the kidney and, at lower levels, in brain, testis, ovary, pancreas and spinal cord. In the kidney, expressed predominantly in the collecting tubular epithelium in the medulla, medullary rays, and periglomerular regions; in the brain, highest levels are found in the cerebellum, choroid plexus, the locus coeruleus, the posterior thalamus and the substantia nigra. Down-regulated in renal tumors compared to normal kidney. Alternatively spliced transcript variants encoding different isoforms have been identified.
  • Cellular localizationMembrane; multi pass membrane protein.
  • Database links
  • Alternative names
    • GON2 antibody
    • long transient receptor potential channel 3 antibody
    • LTrpC 3 antibody
    • LTRPC3 antibody
    • melastatin 2 antibody
    • Melastatin2 antibody
    • MLSN2 antibody
    • transient receptor potential cation channel, subfamily M, member 3 antibody
    see all

Anti-TRPM3 antibody images

  • Anti-TRPM3 antibody (ab32993) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-TRPM3 antibody (ab32993)

ab32993 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"