Overview

  • Product name
    Anti-TRPM5 antibody [EPR7504]
  • Description
    Rabbit monoclonal [EPR7504] to TRPM5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human TRPM5 (UniProt Q9NZQ8).

  • Positive control
    • 293T, Human prostate and BxPC-3 lysates; Mouse heart, mouse spleen, rat heart and rat spleen lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7504
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 131, 98 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals.
    • Tissue specificity
      Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression.
    • Sequence similarities
      Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 9430099A16Rik antibody
      • Long transient receptor potential channel 5 antibody
      • LTrpC-5 antibody
      • LTrpC5 antibody
      • MLSN1 and TRP related gene 1 antibody
      • MLSN1 and TRP related gene 1 protein antibody
      • MLSN1 and TRP-related antibody
      • MLSN1- and TRP-related gene 1 protein antibody
      • MTR1 antibody
      • Novel protein similar to vertebrate transient receptor potential cation channel, subfamily M, member 5 antibody
      • Transient receptor potential cation channel subfamily M member 5 antibody
      • Transient receptor potential cation channel, subfamily M, member 5 antibody
      • Trpm5 antibody
      • TRPM5 transient receptor potential cation channel, subfamily M, member 5 antibody
      • TRPM5_HUMAN antibody
      see all

    Images

    • All lanes : Anti-TRPM5 antibody [EPR7504] (ab154788) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Human prostate lysate
      Lane 3 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 131, 98 kDa

    • All lanes : Anti-TRPM5 antibody [EPR7504] (ab154788) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse spleen lysate
      Lane 3 : Rat heart lysate
      Lane 4 : Rat spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 131, 98 kDa

    References

    This product has been referenced in:
    • Syam N  et al. Glycosylation of TRPM4 and TRPM5 channels: molecular determinants and functional aspects. Front Cell Neurosci 8:52 (2014). WB ; Human . Read more (PubMed: 24605085) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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