Overview

  • Product nameAnti-TRPM7 antibody [EPR4582]
    See all TRPM7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4582] to TRPM7
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide against the residues close to the C-terminus of Human TRPM7.

  • Positive control
    • HuT-78, JAR, and HepG2 cell lysates. HeLa cells and cell lysates.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 213 kDa.Can be blocked with TRPM7 peptide (ab195344).
Flow Cyt 1/220.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionEssential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
    • Involvement in diseaseDefects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
    • Sequence similaritiesIn the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
      In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
      Contains 1 alpha-type protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHAK antibody
      • CHAK1 antibody
      • Channel kinase 1 antibody
      • Channel-kinase 1 antibody
      • Long transient receptor potential channel 7 antibody
      • LTrpC-7 antibody
      • LTrpC7 antibody
      • Transient receptor potential cation channel subfamily M member 7 antibody
      • TRP PLIK antibody
      • TRPM7 antibody
      • TRPM7_HUMAN antibody
      see all

    Anti-TRPM7 antibody [EPR4582] images

    • All lanes : Anti-TRPM7 antibody [EPR4582] (ab109438) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HuT-78 cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 213 kDa
    • Immunofluorescent staining of TRPM7 in HeLa cells using ab109438 at 1/100.
    • Flow Cytometry analysis of HeLa cells labelling TRPM7 with purified ab109438 at a dilution of 1/220 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    References for Anti-TRPM7 antibody [EPR4582] (ab109438)

    This product has been referenced in:
    • Zhou Y  et al. Effects of angiotensin II on transient receptor potential melastatin 7 channel function in cardiac fibroblasts. Exp Ther Med 9:2008-2012 (2015). WB ; Rat . Read more (PubMed: 26136930) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"