Recombinant
RabMAb

Anti-TRPM7 antibody [EPR4582] (ab109438)

Overview

  • Product name
    Anti-TRPM7 antibody [EPR4582]
    See all TRPM7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4582] to TRPM7
  • Host species
    Rabbit
  • Specificity
    The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). The cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPM7 aa 1800-1900 (C terminal).
    (Peptide available as ab195344)

  • Positive control
    • HuT-78, JAR, and HepG2 cell lysates. HeLa cells and cell lysates.
  • General notes

    We have preliminary internal testing data to indicate this antibody may not react with mouse and rat species. Please contact us for more information

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 213 kDa.Can be blocked with TRPM7 peptide (ab195344).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
    • Involvement in disease
      Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
    • Sequence similarities
      In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
      In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
      Contains 1 alpha-type protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALSPDC antibody
      • CHAK antibody
      • CHAK1 antibody
      • Channel kinase 1 antibody
      • Channel-kinase 1 antibody
      • Long transient receptor potential channel 7 antibody
      • LTrpC-7 antibody
      • LTrpC7 antibody
      • Transient receptor potential cation channel subfamily M member 7 antibody
      • TRP PLIK antibody
      • TRPM7 antibody
      • TRPM7_HUMAN antibody
      see all

    Images

    • Anti-TRPM7 antibody [EPR4582] (ab109438) at 1/1000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 213 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-TRPM7 antibody [EPR4582] (ab109438) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HuT-78 cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 213 kDa

    References

    This product has been referenced in:
    • Zhou Y  et al. Effects of angiotensin II on transient receptor potential melastatin 7 channel function in cardiac fibroblasts. Exp Ther Med 9:2008-2012 (2015). WB ; Rat . Read more (PubMed: 26136930) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (Cardiac Fibroblasts)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Cardiac Fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 17 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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