• Product nameAnti-TRPM7 antibody [S74-25]
    See all TRPM7 primary antibodies
  • Description
    Mouse monoclonal [S74-25] to TRPM7
  • SpecificityNo cross reactivity with TRPM6.
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein: LKLPDLKRND YTPDKIIFPQ DESSDLNLQS GNSTKESEAT NSVRLML, corresponding to cytoplasmic C terminal amino acids 1817-1863 of mouse TRPM7.

  • Positive control
    • COS cell lysate transiently transfected with TRPM7.



Our Abpromise guarantee covers the use of ab85016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 212 kDa. Prepare membrane fraction and ensure proteolysis is avoided. After extraction use an SDS buffer, incubate for 15 mins at 37°C before running on SDS-Page.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionEssential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
  • Involvement in diseaseDefects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
  • Sequence similaritiesIn the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
    In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
    Contains 1 alpha-type protein kinase domain.
  • Post-translational
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHAK antibody
    • CHAK1 antibody
    • Channel kinase 1 antibody
    • Channel-kinase 1 antibody
    • Long transient receptor potential channel 7 antibody
    • LTrpC-7 antibody
    • LTrpC7 antibody
    • Transient receptor potential cation channel subfamily M member 7 antibody
    • TRP PLIK antibody
    • TRPM7 antibody
    • TRPM7_HUMAN antibody
    see all

Anti-TRPM7 antibody [S74-25] images

  • ab85016 staining TRPM7 in cultured mouse urothelial cells by Immunocytochemistry. Note that TRPM7 (green) is present in the plasma membrane as well as cytoplasmic vesicles. Nuclei stained blue with DAPI.
    Urothelial cells from mice were fixed with 3.7% formaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 for 10 minutes, and blocked with 3% BSA for 3 hours. The primary antibody was used at a 1/400 dilution and incubated overnight. The secondary antibody was an Alexa Fluor488-conjugated anti-rabbit IgG, used at a 1/1000 dilution, applied for 1 hour at room temperature. Triple washing with PBS was performed between each step.
  • All lanes : Anti-TRPM7 antibody [S74-25] (ab85016) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg

    Predicted band size : 212 kDa
  • ab85016 staining TRPM7 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Overlay histogram showing HeLa cells stained with ab85016 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85016, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-TRPM7 antibody [S74-25] (ab85016)

This product has been referenced in:
  • Zhu Y  et al. Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic reticulum stress-mediated apoptosis. Life Sci 94:37-44 (2014). WB ; Rat . Read more (PubMed: 24220678) »
  • Liu M  et al. TRPM7 channels regulate glioma stem cell through STAT3 and Notch signaling pathways. Cell Signal 26:2773-2781 (2014). Read more (PubMed: 25192910) »

See all 4 Publications for this product

Product Wall

Thank you for your reply and for sending the WB image.

In the WB, I assume lanes 1-3 was the positive control? And lane 4, was your sample?
Since you are detecting the positive control, I would assume that the antibody in general is work...

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Thanks for getting back to me.

I have sent you ab72155 as a free of charge replacement and you should have that by next Wednesday. For your records I gave the free of charge off order number *.

For the other 3 orders you placed with A...

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Would you like me to replace all of the vials that you received, or do you just want one vial replaced and the rest refunded?

Once, I get your reply I will go ahead and get the replacement/s sent to you. There is a wait time of about 1/2 weeks...

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Thank you for your reply.

Yes, all of the antibodies you received came from the same master lot, and also the only lot that we have available at the moment, is that same that you have already received.

I would be happy to replace or r...

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Thank you for providing that information.

Please let me know the catalogue number of the antibody you would wish as a replacement and I will be happy to send that out to you as soon as possible.

I recommended either of the following, ...

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Well, I know its not much consolation, but whatever the band the antibody is detecting, it is really clean.

I think that you would get the same results with the other two vials of the, as you are correct they will all be the same antibody. If ...

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Thank you for your reply and providing that western blot information.

I am also baffled as to what the non-specific bands could be. I checked our notes on this antibody and it should only be recognizing a band at about 200kDa.

I think...

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Thank you for your reply.

I may be misreading your Dot blot (and apologies if I am) but it looks as though you are getting a really intense signal when you probe with 1:100 primary antibody concentration and a 1:1000 secondary antibody concent...

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Thank you for your reply.

The protocol that you are using should be fine, but do you heat your samples before loading? The reason I ask, is because if you heat samples that you wish to probe for membrane proteins, then the heating (above 37C) ...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab85016 in western blotting. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we ca...

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