Overview

  • Product name
  • Description
    Rabbit polyclonal to TRPM8
  • Specificity
    Reacts with residues 278-292 and the C terminus sequence of the human trpm8 protein.
  • Tested applications
    Suitable for: ELISA, IP, IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Guinea pig, Human
  • Immunogen

    Synthetic peptide corresponding to Human TRPM8.
    Sequence:

    RNQLEKYISERTIQD Plus NDLKGLLKEIANKIK

  • Positive control
    • WB: Transfected COS cell lysate; human liver tissue lysate; human hepatocyte lysate. PMID 17510704, prostate cells used as positive control. PC-3 suitable negative control.

Properties

Applications

Our Abpromise guarantee covers the use of ab3243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/2000.
IP Use at an assay dependent concentration.

used at 6 ug/mg cell lysate.

IHC-P 1/10 - 1/2000.
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.
IHC-Fr 1/100. See Abreview.

Target

  • Function
    Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • Tissue specificity
    Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

Images

  • Anti-TRPM8 antibody (ab3243) at 1/750 dilution + TRPM8 transfected Cos7 cell lysate

    Predicted band size : 128 kDa
  • ab3243  staining TRPM8 in human prostate cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • All lanes : Anti-TRPM8 antibody (ab3243) at 1000 cells

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human hepatocyte lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit HRP at 1/1000 dilution

    Predicted band size : 128 kDa

    Image courtesy of an anonymous AbReview

    See Abreview

  • ab3243 staining TRPM8 in murine skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab3243 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Zhao W & Xu H High expression of TRPM8 predicts poor prognosis in patients with osteosarcoma. Oncol Lett 12:1373-1379 (2016). WB, IHC-P ; Human . Read more (PubMed: 27446440) »
  • Liu T  et al. Anti-tumor activity of the TRPM8 inhibitor BCTC in prostate cancer DU145 cells. Oncol Lett 11:182-188 (2016). WB . Read more (PubMed: 26870186) »

See all 15 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
human hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 29 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - whole cell (Hek cells transfected with human TRPM8)
Specification
Hek cells transfected with human TRPM8
Total protein in input
800 µg
Username

Miss. Larisa Maier

Verified customer

Submitted Sep 17 2013

Gracias por contactarnos, le pido disculpas por la espera.

Adjunto al email la datasheet del producto descatalogado ab3243. Al no estar disponible no tengo acceso a la datasheet en formato pdf, por lo que la envío copiada en un documen...

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Thank you for contacting Abcam. Please find the protocol used to test ab3243 in IHC below. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin, HR-1)
Specification
skin, HR-1
Fixative
Methanol
Permeabilization
Yes - 0.4% TritonX100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2011

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab3243 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the e...

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Thank you for your e-mail to Hugh who is away today. I'm very sorry to hear you are still experiencing lots of non specific bands with ab3243 even after the protocol modifications. I would be happy to offer you a replacement vial or refund if you p...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback,...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (prostate cancer)
Specification
prostate cancer
Blocking step
Other as blocking agent for 30 minute(s) · Concentration: 1.2%
Username

Dr. Gabriel Bidaux

Verified customer

Submitted Aug 21 2006

Thank you for your enquiry. I am sorry but we do not have the immunogen peptide for this antibody in our catalogue. It may have been exhausted. Yes, were you to have the two peptides used to raise this antibody synthesized I can see no reason why t...

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1-10 of 19 Abreviews or Q&A

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