Overview

  • Product nameAnti-TRPS1 antibody
    See all TRPS1 primary antibodies
  • Description
    Rabbit polyclonal to TRPS1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1000-1050 of Human TRPS1 (AAG21134.1).

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab125197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionTranscriptional repressor. Binds specifically to GATA sequences and represses expression of GATA-regulated genes at selected sites and stages in vertebrate development. Regulates chondrocyte proliferation and differentiation. Executes multiple functions in proliferating chondrocytes, expanding the region of distal chondrocytes, activating proliferation in columnar cells and supporting the differentiation of columnar into hypertrophic chondrocytes.
  • Tissue specificityUbiquitously expressed in the adult. Found in fetal brain, lung, kidney, liver, spleen and thymus. More highly expressed in androgen-dependent than in androgen-independent prostate cancer cells.
  • Involvement in diseaseDefects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 1 (TRPS1) [MIM:190350]. TRPS1 is an autosomal dominant disorder characterized by craniofacial and skeletal abnormalities. It is allelic with tricho-rhino-phalangeal type 3. Typical features include sparse scalp hair, a bulbous tip of the nose, protruding ears, a long flat philtrum and a thin upper vermilion border. Skeletal defects include cone-shaped epiphyses at the phalanges, hip malformations and short stature.
    Defects in TRPS1 are a cause of tricho-rhino-phalangeal syndrome type 2 (TRPS2) [MIM:150230]. A syndrome that combines the clinical features of trichorhinophalangeal syndrome type 1 and multiple exostoses type 1. Affected individuals manifest multiple dysmorphic facial features including large, laterally protruding ears, a bulbous nose, an elongated upper lip, as well as sparse scalp hair, winged scapulae, multiple cartilaginous exostoses, redundant skin, and mental retardation. Note=A chromosomal aberration resulting in the loss of functional copies of TRPS1 and EXT1 has been found in TRPS2 patients.
    Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 3 (TRPS3) [MIM:190351]. TRPS3 is an autosomal dominant disorder characterized by craniofacial and skeletal abnormalities. It is allelic with tricho-rhino-phalangeal type 1. In TRPS3 a more severe brachydactyly and growth retardation are observed.
  • Sequence similaritiesContains 7 C2H2-type zinc fingers.
    Contains 1 GATA-type zinc finger.
  • Post-translational
    modifications
    Sumoylated. Sumoylation in the repressor domain inhibits the transcription repression activity. Sumoylation on Lys-1201 is the major site. Appears to be sumoylated on multiple sites.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GC79 antibody
    • LGCR antibody
    • Tricho rhino phalangeal syndrome I antibody
    • Tricho-rhino-phalangeal syndrome type I protein antibody
    • Trichorhinophalangeal syndrome I antibody
    • Trichorhinophalangeal syndrome I homolog antibody
    • TRPS 1 antibody
    • trpS1 antibody
    • TRPS1 gene antibody
    • TRPS1_HUMAN antibody
    • Zinc finger protein GC79 antibody
    • Zinc finger transcription factor TRPS 1 antibody
    • Zinc finger transcription factor Trps1 antibody
    see all

Anti-TRPS1 antibody images

  • All lanes : Anti-TRPS1 antibody (ab125197) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 142 kDa


    Exposure time : 3 minutes
  • ab125197 at 1 µg/ml staining TRPS1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab125197 at 6 µg/mg lysate.
    Lane 2; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 30 second exposure.
  • All lanes : Anti-TRPS1 antibody (ab125197) at 1/500 dilution

    Lane 1 : Ha-Ras-transformed EpH4 cells - nuclear extracts
    Lane 2 : EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extracts
    Lane 3 : EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extracts
    Lane 4 : EpH4 (parental) - nuclear extracts
    Lane 5 : EpF-2 - nuclear extracts
    Lane 6 : EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extracts
    Lane 7 : EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extracts
    Lane 8 : EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extracts
    Lane 9 : EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extracts

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 142 kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-TRPS1 antibody (ab125197)

ab125197 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Cell lysate - nuclear (EpH4 (Non tumorogenic mammary epithelial cell line)
Specification EpH4 (Non tumorogenic mammary epithelial cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Jan 28 2014

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