Anti-TRPV3 antibody [N15/39] (ab94582)
Key features and details
- Mouse monoclonal [N15/39] to TRPV3
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-TRPV3 antibody [N15/39]
See all TRPV3 primary antibodies -
Description
Mouse monoclonal [N15/39] to TRPV3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Rat TRPV3 aa 458-474 (C terminal).
Sequence:VSYYRPREGEALPHPLA
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Positive control
- COS-1 cell lysate transiently transfected with TRPV3
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General notes
The clone number has been updated from S15-39 to N15/39, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N15/39 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.1-1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 0.1 - 1 µg/ml.
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ICC/IF |
Use a concentration of 1 - 10 µg/ml.
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Notes |
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Flow Cyt
Use 0.1-1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 0.1 - 1 µg/ml. |
ICC/IF
Use a concentration of 1 - 10 µg/ml. |
Target
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Function
Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. -
Tissue specificity
Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level). -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV3 sub-subfamily.
Contains 3 ANK repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 162514 Human
- Entrez Gene: 246788 Mouse
- Omim: 607066 Human
- SwissProt: Q8NET8 Human
- SwissProt: Q8K424 Mouse
- Unigene: 446255 Human
- Unigene: 347652 Mouse
- Unigene: 75196 Mouse
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Alternative names
- 1110036I10Rik antibody
- MGC124324 antibody
- MGC124325 antibody
see all
Images
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Paraffin-embedded, Bouin's-fixed mouse backskin tissue stained for TRPV3 (green) using ab94582 at 1/100 dilution in immunohistochemical analysis. FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution. Filaggrinlike staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis is observed.
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ice cold methanol-fixed HaCat cells labeling TRPV3 (green) using ab94582 at 1/100 dilution in ICC/IF analysis. FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution. Dotty staining in all cells. Some intermediate filamentlike staining in some cells was observed.
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Overlay histogram showing SH-SY5Y cells stained with ab94582 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94582, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94582 has not yet been referenced specifically in any publications.