Overview

  • Product nameAnti-TRPV4 antibody
    See all TRPV4 primary antibodies
  • Description
    Rabbit polyclonal to TRPV4
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Horse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse TRPV4.

    (Peptide available as ab39471.)

  • Positive control
    • This antibody gave a positive signal in Mouse Brain tissue lysate.
  • General notes

    Although some of our customers have had good results in Human and Rat (89% homology with the immunogen), we do not batch test this antibody in these species.  Due to the polyclonal nature of this antibody, some batches many not work in Human or Rat.

Properties

Applications

Our Abpromise guarantee covers the use of ab39260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-FoFr 1/300.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 98 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionNon-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
  • Tissue specificityFound in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
  • Involvement in diseaseBrachyolmia 3
    Spondylometaphyseal dysplasia Kozlowski type
    Metatropic dysplasia
    Distal spinal muscular atrophy, congenital non-progressive
    Charcot-Marie-Tooth disease 2C
    Scapuloperoneal spinal muscular atrophy
    Spondyloepiphyseal dysplasia Maroteaux type
    Parastremmatic dwarfism
    Digital arthropathy-brachydactyly, familial
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
    Contains 3 ANK repeats.
  • Post-translational
    modifications
    Phosphorylation results in enhancement of its channel function.
  • Cellular localizationCell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • osm 9 like TRP channel 4 antibody
    • Osm-9-like TRP channel 4 antibody
    • OTRPC 4 antibody
    • OTRPC4 antibody
    • Transient receptor potential cation channel subfamily V member 4 antibody
    • Transient receptor potential protein 12 antibody
    • TRP 12 antibody
    • TRP12 antibody
    • TRPV 4 antibody
    • TrpV4 antibody
    • TRPV4_HUMAN antibody
    • Vanilloid receptor like channel 2 antibody
    • Vanilloid receptor like protein 2 antibody
    • Vanilloid receptor related osmotically activated channel antibody
    • Vanilloid receptor-like channel 2 antibody
    • Vanilloid receptor-like protein 2 antibody
    • Vanilloid receptor-related osmotically-activated channel antibody
    • VR 4 antibody
    • VR OAC antibody
    • VR-OAC antibody
    • VR4 antibody
    • VRL 2 antibody
    • VRL-2 antibody
    • VRL2 antibody
    • VROAC antibody
    see all

Anti-TRPV4 antibody images

  • ab39260 staining of TRPV4 -/- (upper panel) and wildtype (lower panel) adult mouse kidney tissue sections, showing good immunostaining in the wildtype tissue and no immunostaining in the TRPV4 -/- tissue. Formalin/PFA-fixed paraffin-embedded sections of mouse kidney tissue were incubated with ab39260 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0. A biotin-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab39260 stained PC12 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab39260, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Anti-TRPV4 antibody (ab39260) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 25 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 98 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,54 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • IHC-FoFr image of TRPV4 staining on Rat DRG sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat. The image shows DRG neurons (left), nerve taken at the level of the root (right)

    See Abreview

  • All lanes : Anti-TRPV4 antibody (ab39260) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from MEF cells
    Lane 2 : Whole cell lysate prepared from F9 cells
    Lane 3 : Whole cell lysate prepared from GC-1 cells
    Lane 4 : Whole cell lysate prepared from NIH-3T3 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 98 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-TRPV4 antibody (ab39260)

This product has been referenced in:
  • Hdud IM  et al. Effects of cyclic equibiaxial mechanical stretch on a-BK and TRPV4 expression in equine chondrocytes. Springerplus 3:59 (2014). WB ; Horse . Read more (PubMed: 24516787) »
  • Egbuniwe O  et al. TRPA1 and TRPV4 activation in human odontoblasts stimulates ATP release. J Dent Res 93:911-7 (2014). WB ; Human . Read more (PubMed: 25062738) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Aortic endothelial cell)
Permeabilization No
Gating Strategy size and granularity
Specification Aortic endothelial cell
Preparation Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with 0.05% Trypsin-EDTA
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation Paraformaldehyde
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Submitted Dec 30 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 05 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin
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Submitted Oct 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 200 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Sample Rat Tissue lysate - whole (bladder)
Specification bladder
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Jul 18 2013

Thank you for contacting us. The first image on our website did show a band at ˜ 100 kD as predicted.
There is also a different image from a customer Abreview with a band ˜125 kDa in 4 different mouse cell lines. However, there are published report...

Read More

Thank you for contacting us. For blocking we typically test our antibodies with 3% BSA although you may use 5% nonfat milk if that is your preference.

Regarding the concentration for WB:

ab11370 use at 1/8000

ab39262 use 1-2 ...

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Thank you very much for your call today and for letting us know about the problem with the vial of this antibody.

I'm sending a free of charge replacement vial on the order ***, which should arrive tomorrow. Please keep me updated about the r...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse cell lines)
Loading amount 20 µg
Specification Mouse cell lines
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

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Verified customer

Submitted Feb 23 2012

Thank you for your email.
The co-IP of membrane proteins is a challenge.
I would not recommend using the RIPA buffer as the buffer is very harsh and partly denatures the proteins due to the anionic detergent SDS.
The somewhat milder buff...

Read More

Thank you for your email.
I'm so sorry for my oversight. Thank you so much for reminding me.
Here is the RIPA buffer composition:
RIPA buffer (radio immuno precipitation assay) buffer:
RIPA buffer contains the ionic detergent sodium...

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"