The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
Involvement in disease
Brachyolmia 3 Spondylometaphyseal dysplasia Kozlowski type Metatropic dysplasia Distal spinal muscular atrophy, congenital non-progressive Charcot-Marie-Tooth disease 2C Scapuloperoneal spinal muscular atrophy Spondyloepiphyseal dysplasia Maroteaux type Parastremmatic dwarfism Digital arthropathy-brachydactyly, familial
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily. Contains 3 ANK repeats.
Phosphorylation results in enhancement of its channel function.
Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
ab94868 staining TRPV4 in cultured hippocampal slices of rat brain by Immunohistochemistry - PFA fixed sections.
Slice cultures attached to the PTFE membrane were fixed with 4% paraformaldehyde in PBS for 20 minutes at room temperature. They were permeabilized with 1% Triton X-100 in PBS overnight at 4 °C, then blocked with 3% bovine serum albumin (BSA) in PBS and incubated overnight at 4 °C with ab94868 (Red) at a 1/500 dilution. Following washes with PBS and blocking with 3% BSA, tissue was incubated for 1 hour at room temperature with Alexa Fluor 568-conjugated goat anti-rabbit antibody diluted 1/500 in blocking solution. Tissue was washed in PBS, mounted with DAPI.Blue: DAPI. Green: anti-Map2.Red: ab94868.
Western blot - TRPV4 antibody (ab94868)
Anti-TRPV4 antibody (ab94868) at 1 µg/ml + HT1080 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 91 kDa Gel concentration: 6-18%
Alkozi HA et al. TRPV4 Stimulation Induced Melatonin Secretion by Increasing Arylalkymine N-acetyltransferase (AANAT) Protein Level. Int J Mol Sci18:N/A (2017).
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