Overview

  • Product nameAnti-TRPV5 antibody
    See all TRPV5 primary antibodies
  • Description
    Mouse monoclonal to TRPV5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MGGFLPKAEG PGSQLQKLLP SFLVREQDWD QHLDKLHMLQ QKRILESPLL RASKENDLSV LRQLLLDCTC DVRQRGALGE TALHIAALYD NLEAALVLME , corresponding to amino acids 1-101 of Human TRPV5

Properties

Applications

Our Abpromise guarantee covers the use of ab57630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 20547482

Target

  • FunctionConstitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
  • Tissue specificityExpressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum.
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily.
    Contains 5 ANK repeats.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationApical cell membrane. Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium transport protein 2 antibody
    • Calcium transporter 2 antibody
    • CAT 2 antibody
    • CAT2 antibody
    • ECAC 1 antibody
    • ECaC antibody
    • ECaC1 antibody
    • Epithelial calcium channel 1 antibody
    • Osm 9 like TRP channel 3 antibody
    • Osm-9-like TRP channel 3 antibody
    • OTRPC 3 antibody
    • OTRPC3 antibody
    • Transient receptor potential cation channel subfamily V member 5 antibody
    • TRPV 5 antibody
    • TrpV5 antibody
    • TRPV5_HUMAN antibody
    see all

Anti-TRPV5 antibody images

  • Anti-TRPV5 antibody (ab57630) + immunogen

    Predicted band size : 83 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Sandwich ELISA. The detection limit for recombinant tagged TRPV5 is approximately 0.03ng/ml as a capture antibody

References for Anti-TRPV5 antibody (ab57630)

This product has been referenced in:
  • Chamoux E  et al. TRPV-5 Mediates a Receptor Activator of NF-{kappa}B (RANK) Ligand-induced Increase in Cytosolic Ca2+ in Human Osteoclasts and Down-regulates Bone Resorption. J Biol Chem 285:25354-62 (2010). ICC/IF ; Human . Read more (PubMed: 20547482) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab57630.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"