Overview

  • Product name
  • Description
    Goat polyclonal to TRPV5
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-SHRGWEILRQNT

    , corresponding to amino acids 697-708 of Human TRPV5 (NP_062815.2).

  • Positive control
    • Human Pancreas lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 83 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating.
    • Tissue specificity
      Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum.
    • Sequence similarities
      Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV5 sub-subfamily.
      Contains 5 ANK repeats.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Apical cell membrane. Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity). The expression of the glycosylated form in the cell membrane is increased in the presence of WNK3.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium transport protein 2 antibody
      • Calcium transporter 2 antibody
      • CAT 2 antibody
      • CAT2 antibody
      • ECAC 1 antibody
      • ECaC antibody
      • ECaC1 antibody
      • Epithelial calcium channel 1 antibody
      • Osm 9 like TRP channel 3 antibody
      • Osm-9-like TRP channel 3 antibody
      • OTRPC 3 antibody
      • OTRPC3 antibody
      • Transient receptor potential cation channel subfamily V member 5 antibody
      • TRPV 5 antibody
      • TrpV5 antibody
      • TRPV5_HUMAN antibody
      see all

    Images

    • Anti-TRPV5 antibody (ab77351) at 1 µg/ml + Human pancreas lysate in RIPA buffer at 35 µg

      Predicted band size : 83 kDa
      Observed band size : 83 kDa

    References

    ab77351 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77351.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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