Overview

  • Product name
  • Description
    Rabbit polyclonal to TRRAP
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan, Medaka fish, Platypus
  • Immunogen

    Synthetic peptide from a region between residue 3275 and 3325 of human TRRAP, AAD04629.1.

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      TRRAP (TRansformation/tRanscription domain Associated Protein) is an adapter protein that is found in a number of multiprotein chromatin complexes with histone acetyltransferase (HAT) activity. These complexes include PCAF, TFTC-HAT, TIP60 HAT, STAGA, and BAF53 complexes. TRRAP is thought to be responsible for the concerted and context-dependent recruitment of these complexes to chromatin during transcription, replication and DNA repair. TRRAP plays a key role in transcriptional activation by c-Myc, p53/TP53, E2F1 and E2F4.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • 350/400 kDa PCAF associated factor antibody
      • PAF 350/400 antibody
      • PAF 400 antibody
      • PAF350/400 antibody
      • PAF400 antibody
      • STAF 40 antibody
      • STAF40 antibody
      • TR AP antibody
      • Tra 1 antibody
      • Tra1 antibody
      • Tra1 homolog antibody
      • Transactivation / transformation domain associated protein antibody
      • Transactivation/transformation domain associated protein antibody
      • Transformation/transcription domain associated protein antibody
      • TRAP antibody
      see all

    Images

    • Immunoprecipitation/ Western Blot of TRRAP.
      Lane 1: ab72509 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      Another antibody to TRRAP was used at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    References

    ab72509 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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