Anti-TRRAP antibody - ChIP Grade (ab73546)

Overview

  • Product nameAnti-TRRAP antibody - ChIP Grade
    See all TRRAP primary antibodies
  • Description
    Rabbit polyclonal to TRRAP - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 3800 to the C-terminus of Human TRRAP.

  • Positive control
    • This antibody gave a positive signal in the following nuclear lysate preparations: HeLa; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab73546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa).
ChIP Use 5 µg for 25 µg of chromatin.

Target

  • RelevanceTRRAP (TRansformation/tRanscription domain Associated Protein) is an adapter protein that is found in a number of multiprotein chromatin complexes with histone acetyltransferase (HAT) activity. These complexes include PCAF, TFTC-HAT, TIP60 HAT, STAGA, and BAF53 complexes. TRRAP is thought to be responsible for the concerted and context-dependent recruitment of these complexes to chromatin during transcription, replication and DNA repair. TRRAP plays a key role in transcriptional activation by c-Myc, p53/TP53, E2F1 and E2F4.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 350/400 kDa PCAF associated factor antibody
    • PAF 350/400 antibody
    • PAF 400 antibody
    • PAF350/400 antibody
    • PAF400 antibody
    • STAF 40 antibody
    • STAF40 antibody
    • TR AP antibody
    • Tra 1 antibody
    • Tra1 antibody
    • Tra1 homolog antibody
    • Transactivation / transformation domain associated protein antibody
    • Transactivation/transformation domain associated protein antibody
    • Transformation/transcription domain associated protein antibody
    • TRAP antibody
    see all

Anti-TRRAP antibody - ChIP Grade images

  • All lanes : Anti-TRRAP antibody - ChIP Grade (ab73546) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 438 kDa
    Observed band size : 438 kDa
    Additional bands at : 150 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab73546 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach for both the active and heterochromatic loci). Primers are located in the first kb of the transcribed region.

References for Anti-TRRAP antibody - ChIP Grade (ab73546)

ab73546 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Flag-tagged TRRAP)
Loading amount 0.05 µg
Specification Flag-tagged TRRAP
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"