Overview

  • Product nameAnti-TRUB1 antibody
    See all TRUB1 primary antibodies
  • Description
    Mouse polyclonal to TRUB1
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (NP_631908.1)

  • Positive control
    • WB: mammalian transfected lysate. ICC/IF: HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay be responsible for synthesis of pseudouridine from uracil in transfer RNAs.
  • Tissue specificityHighly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen.
  • Sequence similaritiesBelongs to the pseudouridine synthase TruB family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Probable tRNA pseudouridine synthase 1 antibody
    • PUS4 antibody
    • TruB pseudouridine (psi) synthase homolog 1 (E. coli) antibody
    • Trub1 antibody
    • TRUB1_HUMAN antibody
    see all

Anti-TRUB1 antibody images

  • All lanes : Anti-TRUB1 antibody (ab72866) at 1/500 dilution

    Lane 1 : TRUB1-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 37 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells using protein A affiinity purified ab72866 TRUB1 at 10 ug/ml.

References for Anti-TRUB1 antibody (ab72866)

ab72866 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry regarding ab72866. I can confirm that this antibody is affinity purified and we have just removed the purification note in the application section to avoid any further confusion. It has only been tested for Western bl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"