Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651)

Overview

  • Product name
    Anti-Trypanosoma brucei phosphoglycerate kinase antibody
  • Description
    Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
  • Tested applications
    Suitable for: ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with Trypanosoma brucei
  • Immunogen

    Synthetic peptide derived from the C terminal domain of Trypanosoma brucei phosphoglycerate kinase.

Properties

Applications

Our Abpromise guarantee covers the use of ab89651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 55 kDa.

Target

  • Relevance
    Belongs to the phosphoglycerate kinase family.
  • Alternative names
    • PGK A allele 2 antibody
    • Phosphoglycerate kinase A antibody
    • Trypanosoma brucei PGK antibody

Anti-Trypanosoma brucei phosphoglycerate kinase antibody images

  • All lanes : Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) at 1/200 dilution (in TBS + 5% milk for 12 hours at 4°C)

    Lane 1 : Whole cell lysate of Trypanosoma brucei - procyclic form
    Lane 2 : Whole cell lysate of Trypanosoma brucei - bloodstream

    Lysates/proteins at 20000000 cells per lane.

    Secondary
    A biotin-conjugated Goat anti-rabbit IgG polyclonal at 1/200 dilution

    Predicted band size : 55 kDa
    Observed band size : 65,75 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Susan Rouse

    See Abreview

References for Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651)

ab89651 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Trypanosoma brucei Cell lysate - whole cell (Trypanosoma brucei procyclic form and bloodstream)
Loading amount
2e+007 cells
Specification
Trypanosoma brucei procyclic form and bloodstream
Gel Running Conditions
Reduced Denaturing (10% separating 4% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Susan Rouse

Verified customer

Submitted Aug 26 2010

Application
Immunocytochemistry
Sample
Trypanosoma brucei Cultured Cells (Blood stream and procyclic forms)
Specification
Blood stream and procyclic forms
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 37°C
Username

Dr. Susan Rouse

Verified customer

Submitted Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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