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Trypsin Activity Assay Kit (Colorimetric) (ab102531) is an assay where trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.
The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
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Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either amino acid is followed by proline.
Trypsin is commonly used in numerous biotechnological processes:
• Trypsinization or trypsin proteolysis: digestion of proteins into peptides for mass spectrometry analysis.
• Breakdown casein in break milk.
• Production of hypoallergenic food where proteases break down specific allergenic proteins into nonallergenic peptides (for example, production of hypoallergenic baby food from cow’s milk).
|Chymotrypsin Inhibitor (TPCK,10 mM)||White||1 x 100 units|
|p-NA Standard (2 mM)||Yellow||1 x 400µl|
|Positive Control ( lyophilized)||Blue||1 vial|
|Trypsin Assay Buffer||WM||1 x 25ml|
|Trypsin Inhibitor (TLCK, 20 mM)||Purple||1 x 100µl|
|Trypsin Substrate (in DMSO)||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab102531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Trypsin standard curve measured in duplicates ((+/- SD), background subtracted) after 60 minutes incubation.
Trypsin levels were kinetically measured in commercially purchased trypsin (1x); background signal subtracted (duplicates +/- SD).