Overview

  • Product nameTrypsin Activity Assay Kit (Colorimetric)
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 10 mU/well
  • Range
    10 mU/well - 100 mU/well
  • Assay time
    2h 00m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Product overview

    Trypsin Activity Assay Kit (Colorimetric) (ab102531) is an assay where trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.

    The kit detects 10-100 mU (p-NA unit) trypsin in various samples.

     


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either amino acid is followed by proline.
    Trypsin is commonly used in numerous biotechnological processes:
    • Trypsinization or trypsin proteolysis: digestion of proteins into peptides for mass spectrometry analysis.
    • Breakdown casein in break milk.
    • Production of hypoallergenic food where proteases break down specific allergenic proteins into nonallergenic peptides (for example, production of hypoallergenic baby food from cow’s milk).

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformMicroplate

Properties

  • FunctionHas activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • Involvement in diseaseDefects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Alternative names
    • Alpha-trypsin chain 2
    • Beta-trypsin
    • Cationic trypsinogen
    • Digestive zymogen
    • Nonfunctional trypsin 1
    • Prss1
    • Serine protease 1
    • TCR V beta 4.1
    • TRP1
    • TRY1
    • TRY1_HUMAN
    • TRY4
    • TRYP1
    • Trypsin I
    • Trypsinogen 1
    • Trypsinogen A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab102531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Trypsin Activity Assay Kit (Colorimetric) images

  • Trypsin standard curve measured in duplicates ((+/- SD), background subtracted) after 60 minutes incubation.

  • Trypsin levels were kinetically measured in commercially purchased trypsin (1x); background signal subtracted (duplicates +/- SD).

  • Example of Trypsin sample timeline obtained using ab102531

Protocols

References for Trypsin Activity Assay Kit (Colorimetric) (ab102531)

This product has been referenced in:
  • Lebleu VS  et al. Identification of human epididymis protein-4 as a fibroblast-derived mediator of fibrosis. Nat Med 19:227-31 (2013). Functional Studies . Read more (PubMed: 23353556) »

See 1 Publication for this product

Product Wall

There is ˜8-10 mM calcium in the buffer. Typically trypsin is not inhibited by this.



The kit detects 10-100 mU (p-NA unit) trypsin in various samples. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ = 405 nm.

One unit is defined as the amount of trypsin that cleaves ...

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These 2 kit buffers have different components and pH. They cannot be used interchangeably and it is not recommended to perform the extraction with PBS.

Thank you very much for letting me know the kit reached you safely.

I apologise for the inconvenience this problem has caused you. If I can be of any further assistance, please do not hesitate to let me know.

Otherwise, I wish you al...

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Thank you for contacting us yesterday and for reporting the problems encountered with the Trypsin Activity Assay Kit (ab102531).

As discussed over the phone, I have checked into why you have received ˜20 uL of p-NA standard instead of the expe...

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The Chymotrypsin inhibitor will inhibit the chymotrypsin activity and therefore any colour change in the samples can be completely attributed to just the trypsin activity.

Thank you for contacting Abcam earlier today.

Please find the definitions that we discussed below:

TAME - of p-toluene-sulfonyl-L-arginine methyl ester

BAEE - Nα-benzoyl-L-arginine ethyl ester

If there is anyth...

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Yes, it may be that something in the serum sample was inhibiting the reaction and slowing it down.

Another thought - If the serum has some trypsinogen in it also, it can take some time for that to get converted into trypsin which will increas...

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Merci de nous avoir contactés.

Le kit ab102531 a été développé pour la détection de la trypsine.
Nous avons à notre catalogue un kit pour mesurer la quantité totale de protéase...

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Thank you for contacting Abcam regarding ab102531.


I have confirmed with the laboratory that no, the trypsin will convert the trypsinogen on its own if you preincubate the cell lysate at RT. So after you extract the cells with the assay b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"