Anti-Trypsin antibody [2C4] (ab17263)

Overview

  • Product nameAnti-Trypsin antibody [2C4]
    See all Trypsin primary antibodies
  • Description
    Mouse monoclonal [2C4] to Trypsin
  • SpecificityThis antibody is specific for human trypsin, trypsinogen and trypsin in complex with alpha 1 antitrypsin.
  • Tested applicationsSuitable for: Sandwich ELISA, ELISA, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Full length native protein purified from human pancreatic tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab17263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be used as a detection antibody in conjugation with ab17262.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 24 kDa.

Target

  • FunctionHas activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • Involvement in diseaseDefects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-trypsin chain 2 antibody
    • Anionic trypsinogen antibody
    • Beta trypsin antibody
    • Beta-trypsin antibody
    • Brain trypsinogen antibody
    • Cationic trypsinogen antibody
    • Digestive zymogen antibody
    • Mesotrypsin antibody
    • Mesotrypsinogen antibody
    • MGC111183 antibody
    • MGC120174 antibody
    • MGC120175 antibody
    • MGC149362 antibody
    • MTG antibody
    • Nonfunctional trypsin 1 antibody
    • Pancreatic trypsinogen III antibody
    • Protease serine 1 (trypsin 1) antibody
    • Protease Serine 1 antibody
    • Protease serine 2 (trypsin 2) antibody
    • Protease serine 2 antibody
    • Protease serine 2 preproprotein antibody
    • Protease serine 3 (mesotrypsin) antibody
    • Protease serine 3 antibody
    • Protease serine 4 (trypsin 4 brain) antibody
    • Prss1 antibody
    • PRSS2 antibody
    • PRSS3 antibody
    • PRSS4 antibody
    • Serine protease 1 antibody
    • Serine protease 2 antibody
    • Serine protease 3 antibody
    • Serine protease 4 antibody
    • T9 antibody
    • TRP1 antibody
    • TRY1 antibody
    • TRY1_HUMAN antibody
    • TRY2 antibody
    • TRY3 antibody
    • TRY4 antibody
    • TRY8 antibody
    • TRYP1 antibody
    • TRYP2 antibody
    • Trypsin 1 antibody
    • Trypsin 2 antibody
    • Trypsin 3 antibody
    • Trypsin I antibody
    • Trypsin II antibody
    • Trypsin III antibody
    • Trypsin IV antibody
    • Trypsinogen 1 antibody
    • Trypsinogen 2 antibody
    • Trypsinogen 4 antibody
    • Trypsinogen 5 antibody
    • Trypsinogen A antibody
    • Trypsinogen, anionic antibody
    • Trypsinogen, cationic antibody
    • Ttrypsinogen IV antibody
    see all

Anti-Trypsin antibody [2C4] images

  • All lanes : Anti-Trypsin antibody [2C4] (ab17263) at 5 µg/ml

    Lane 1 : Natural human Trypsin protein (ab78527) at 1 µg
    Lane 2 : Natural human Trypsin protein (ab78527) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Anti-Trypsin antibody [2C4] (ab17263)

ab17263 has not yet been referenced specifically in any publications.

Product Wall

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Thanks for your inquiry. I am sorry but we do not have a recommended dilution guideline for the sandwich setup. The originator has done some experimental work and we know it works, but we have not optimized a setup. I am sorry I cannot be of more he...

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