This antibody gave a positive signal in the following tissue lysates:
Rat Brain (data not shown)
Rat Serotoninergic nucleus (5HT)
Mouse Serotoninergic nucleus (5HT)
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
IHC image of Tryptophan Hydroxylase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46757, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Tryptophan Hydroxylase antibody (ab46757)
All lanes : Anti-Tryptophan Hydroxylase antibody (ab46757) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Serotoninergic nucleus (5HT) Rat Tissue Lysate Lane 4 : Serotoninergic nucleus (5HT) Mouse Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution