Recombinant
RabMAb

Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954)

Overview

  • Product name
    Anti-Tryptophan Hydroxylase antibody [EP1311Y]
    See all Tryptophan Hydroxylase primary antibodies
  • Description
    Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human Tryptophan Hydroxylase

  • Positive control
    • THP-1 cell lysate and human urinary bladder carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.

Target

  • Tissue specificity
    Isoform 2 seems to be less widely expressed than isoform 1.
  • Pathway
    Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • Sequence similarities
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Indoleacetic acid 5 hydroxylase antibody
    • L tryptophan hydroxylase antibody
    • MGC119994 antibody
    • TPH 1 antibody
    • TPH antibody
    • TPH1 antibody
    • TPH1_HUMAN antibody
    • TPRH antibody
    • TRPH antibody
    • Tryptophan 5 hydroxylase 1 antibody
    • Tryptophan 5 monooxygenase 1 antibody
    • Tryptophan 5 monooxygenase antibody
    • Tryptophan 5-hydroxylase 1 antibody
    • Tryptophan 5-monooxygenase 1 antibody
    • Tryptophan hydroxylase 1 antibody
    see all

Anti-Tryptophan Hydroxylase antibody [EP1311Y] images

  • Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954) at 1/500 dilution + THP-1 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • ab52954 at 1/50 dilution staining Tryptophan Hydroxylase in human urinary bladder carcinoma by Immunohistochemistry, Paraffin embedded tissue.
  • Overlay histogram showing HeLa cells stained with ab52954 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52954, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954)

This product has been referenced in:
  • Acosta-Andrade C  et al. A novel role for antizyme inhibitor 2 as a regulator of serotonin and histamine biosynthesis and content in mouse mast cells. Amino Acids 48:2411-21 (2016). Read more (PubMed: 27084713) »
  • Valdés-Fuentes M  et al. Effect of daytime-restricted feeding in the daily variations of liver metabolism and blood transport of serotonin in rat. Physiol Rep 3:N/A (2015). WB, IHC . Read more (PubMed: 25948822) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 08 2016

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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DISCOUNT CODE: ***
Expiration date: March 1, 2013
Value: $337

I am very pleased to hear you would like to accept our offer and test ab52954 with baboon samples. This code will give you $337 off your next order before the expiration ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Raphe Nucleus)
Specification
Brain, Raphe Nucleus
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0.1%
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 26 2012

Thank you for contacting us. The IHC-FrFl Abreview was submitted to us by one of our customer few months back. We had tried to troubleshoot the protocol however we were not able to resolve the issue. Because we do not have the IHC-Fr tested dat...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Hindbrain)
Specification
Hindbrain
Username

Abcam user community

Verified customer

Submitted Apr 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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