Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574)
Key features and details
- Rabbit polyclonal to Tryptophan Hydroxylase/TPH (phospho S260)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody
See all Tryptophan Hydroxylase/TPH primary antibodies -
Description
Rabbit polyclonal to Tryptophan Hydroxylase/TPH (phospho S260) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Rat Tryptophan Hydroxylase/TPH (phospho S260).
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Positive control
- Rat brain stem lysate, human dorsal Raphe nucleus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Ab30574 was affinity purified via sequential chromatography on phospho and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
Target
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Tissue specificity
Isoform 2 seems to be less widely expressed than isoform 1. -
Pathway
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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Database links
- Entrez Gene: 24848 Rat
- SwissProt: P09810 Rat
- Unigene: 94788 Rat
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Alternative names
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
Images
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All lanes : Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574) at 1/1000 dilution
Lane 1 : rat brain stem lysate (control)
Lane 2 : rat brain stem lysate, incubated with S260 phosphopeptide (negative control)
Lane 3 : rat brain stem lysate, incubated with corresponding non phosphopeptide.
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: <43 kDa, 48 kDa, 59 kDa. We are unsure as to the identity of these extra bands.
In lane 2, the labeling is specifically blocked by the S260 phosphopeptide used as antigen.
In lane 3, the corresponding nonphosphopeptide did not block the immunolabeling.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab30574 has been referenced in 4 publications.
- Herrera-Martínez AD et al. Effect of the Tryptophan Hydroxylase Inhibitor Telotristat on Growth and Serotonin Secretion in 2D and 3D Cultured Pancreatic Neuroendocrine Tumor Cells. Neuroendocrinology 110:351-363 (2020). PubMed: 31319410
- Damen R et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One 8:e62607 (2013). PubMed: 23638125
- Chin A et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol 302:G397-405 (2012). WB ; Human . PubMed: 22038827
- Haugen M et al. Differential signal pathway activation and 5-HT function: the role of gut enterochromaffin cells as oxygen sensors. Am J Physiol Gastrointest Liver Physiol 303:G1164-73 (2012). WB ; Human . PubMed: 22936271