Anti-Tryptophan Hydroxylase (phospho S260) antibody (ab30574)

Overview

  • Product nameAnti-Tryptophan Hydroxylase (phospho S260) antibody
    See all Tryptophan Hydroxylase primary antibodies
  • Description
    Rabbit polyclonal to Tryptophan Hydroxylase (phospho S260)
  • SpecificityAb30574 recognises Tryptophan Hydroxylase (phospho S260).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser260 of rat Tryptophan Hydroxylase.

  • Positive control
    • Rat brain stem lysate, human dorsal Raphe nucleus.

Properties

Applications

Our Abpromise guarantee covers the use of ab30574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Tissue specificityIsoform 2 seems to be less widely expressed than isoform 1.
  • PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Indoleacetic acid 5 hydroxylase antibody
    • L tryptophan hydroxylase antibody
    • MGC119994 antibody
    • TPH 1 antibody
    • TPH antibody
    • TPH1 antibody
    • TPH1_HUMAN antibody
    • TPRH antibody
    • TRPH antibody
    • Tryptophan 5 hydroxylase 1 antibody
    • Tryptophan 5 monooxygenase 1 antibody
    • Tryptophan 5 monooxygenase antibody
    • Tryptophan 5-hydroxylase 1 antibody
    • Tryptophan 5-monooxygenase 1 antibody
    • Tryptophan hydroxylase 1 antibody
    see all

Anti-Tryptophan Hydroxylase (phospho S260) antibody images

  • All lanes : Anti-Tryptophan Hydroxylase (phospho S260) antibody (ab30574) at 1/1000 dilution

    Lane 1 : rat brain stem lysate (control)
    Lane 2 : rat brain stem lysate, incubated with S260 phosphopeptide (negative control)
    Lane 3 : rat brain stem lysate, incubated with corresponding non phosphopeptide.

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,59 kDa,<43 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Tryptophan Hydroxylase (phospho S260) antibody (ab30574)

This product has been referenced in:
  • Damen R  et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One 8:e62607 (2013). Read more (PubMed: 23638125) »
  • Chin A  et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol 302:G397-405 (2012). WB ; Human . Read more (PubMed: 22038827) »

See all 3 Publications for this product

Product Wall

The sequence of the antigen for this antibody is conserved only in TPH1, however it may still recognize TPH2 although there is not 100 % homology with the TPH2 sequence. The ability of this antibody to recognize TPH2 specifically has not yet been dire...

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Thank you for your enquiry. I have been in touch with the source of this antibody and I can confirm that the lysate used for that blot was made from untreated rat brain. I hope this information helps, please do not hesitate to contact us if you ...

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This antibody is specific for TPH1. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"