Anti-Tryptophan rich protein antibody (ab77053)

Overview

  • Product nameAnti-Tryptophan rich protein antibody
    See all Tryptophan rich protein primary antibodies
  • Description
    Mouse monoclonal to Tryptophan rich protein
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment: PSFSSFMSRV LQKDAEQESQ MRAEIQDMKQ ELSTVNMMDE FARYARLERK NKMTDKLKTH VKARTAQLAK IK, corresponding to amino acids 29 - 101 of Human Tryptophan rich protein (NP_004618) with a 26 kDa tag

  • Positive control
    • HeLa cell lysate; immunogen

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Tryptophan rich protein is approximately 1 ng/ml.

Target

  • RelevanceThis gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues. Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients has been restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Down syndrom congenital heart disease.
  • Cellular localizationNucleus. Membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • CHD5 antibody
    • Congenital heart disease 5 gene antibody
    • Congenital heart disease 5 protein antibody
    • FLJ51808 antibody
    • Tryptophan rich basic protein antibody
    • Tryptophan rich protein, congenital heart disease 5 protein antibody
    • WRB antibody
    see all

Anti-Tryptophan rich protein antibody images

  • Anti-Tryptophan rich protein antibody (ab77053) at 1/500 dilution + recombinant tagged Tryptophan rich protein (the immunogen) at 0.2 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 19 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Anti-Tryptophan rich protein antibody (ab77053) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 19 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • Capture ELISA using ab77053 and recombinant tagged Tryptophan rich protein

References for Anti-Tryptophan rich protein antibody (ab77053)

ab77053 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77053.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"