Overview

  • Product nameAnti-Tryptophanyl tRNA synthetase antibody
    See all Tryptophanyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Tryptophanyl tRNA synthetase
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human): Tryptophanyl tRNA synthetase

  • Positive control
    • human normal breast tissue. HEK293 cell line.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF 1/50 - 1/1000.
IP Use at an assay dependent concentration.

Target

  • FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Post-translational
    modifications
    Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma 2 antibody
    • GAMMA-2 antibody
    • Gamma2 antibody
    • hWRS antibody
    • IFI 53 antibody
    • IFI53 antibody
    • IFP 53 antibody
    • IFP53 antibody
    • Interferon induced protein 53 antibody
    • Interferon-induced protein 53 antibody
    • SYWC_HUMAN antibody
    • T2-TrpRS antibody
    • TrpRS antibody
    • Tryptophan tRNA ligase, cytoplasmic antibody
    • Tryptophan tRNA ligase 1 cytoplasmic antibody
    • Tryptophan tRNA ligase antibody
    • Tryptophan--tRNA ligase antibody
    • Tryptophanyl tRNA synthetase antibody
    • Tryptophanyl tRNA synthetase cytoplasmic antibody
    • WARS antibody
    • WARS protein antibody
    • WRS antibody
    see all

Anti-Tryptophanyl tRNA synthetase antibody images

  • Anti-Tryptophanyl tRNA synthetase antibody (ab31536) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab31536 staining Tryptophanyl tRNA synthetase in HeLa cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Tryptophanyl tRNA synthetase antibody (ab31536)

This product has been referenced in:
  • Norris EL  et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics 15:3297-3320 (2016). WB ; Human . Read more (PubMed: 27451424) »
  • Al-Gubory KH  et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction 147:599-614 (2014). WB, IHC-P ; Sheep . Read more (PubMed: 24446454) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"