The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
1/50 - 1/1000.
Use at an assay dependent concentration.
FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
Post-translational modificationsProteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
ab31536 staining Tryptophanyl tRNA synthetase in HeLa cells by Immunocytochemistry/ Immunofluorescence.
References for Anti-Tryptophanyl tRNA synthetase antibody (ab31536)
This product has been referenced in:
Al-Gubory KH et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction147:599-614 (2014).
Read more (PubMed: 24446454) »
Vellaichamy A et al. Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases. PLoS One4:e7075 (2009).
Read more (PubMed: 19763266) »