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Product name Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] See all Tryptophanyl tRNA synthetase primary antibodies
Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase
Tested applications Suitable for:
WB, IHC-P, Flow Cyt more details Unsuitable for:
ICC or IP
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Tryptophanyl tRNA synthetase.
HeLa, JAR, 293T and L6 cell lysates; Human breast tissue
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR3423
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
1/500 - 1/1000. Antigen retrieval is recommended.
1/10 - 1/100.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes Is unsuitable for ICC or IP.
Function Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
Sequence similarities Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
Post-translational modifications Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
Cellular localization Cytoplasm.
Information by UniProt
Gamma 2 antibody
Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] images
Western blot - Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213)
All lanes : Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : JAR cell lysate Lane 3 : 293T cell lysate Lane 4 : L6 cell lysate Lysates/proteins at 10 µg per lane. Predicted band size : 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213)
ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase in paraffin-embedded Human breast tissue by Immunohistochemistry.
References for Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"