Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213)

Overview

  • Product nameAnti-Tryptophanyl tRNA synthetase antibody [EPR3423]
    See all Tryptophanyl tRNA synthetase primary antibodies
  • Description
    Rabbit monoclonal [EPR3423] to Tryptophanyl tRNA synthetase
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Tryptophanyl tRNA synthetase.

  • Positive control
    • HeLa, JAR, 293T and L6 cell lysates; Human breast tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
IHC-P 1/500 - 1/1000. Antigen retrieval is recommended.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionIsoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
    • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
      Contains 1 WHEP-TRS domain.
    • Post-translational
      modifications
      Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gamma 2 antibody
      • GAMMA-2 antibody
      • Gamma2 antibody
      • hWRS antibody
      • IFI 53 antibody
      • IFI53 antibody
      • IFP 53 antibody
      • IFP53 antibody
      • Interferon induced protein 53 antibody
      • Interferon-induced protein 53 antibody
      • SYWC_HUMAN antibody
      • T2-TrpRS antibody
      • TrpRS antibody
      • Tryptophan tRNA ligase, cytoplasmic antibody
      • Tryptophan tRNA ligase 1 cytoplasmic antibody
      • Tryptophan tRNA ligase antibody
      • Tryptophan--tRNA ligase antibody
      • Tryptophanyl tRNA synthetase antibody
      • Tryptophanyl tRNA synthetase cytoplasmic antibody
      • WARS antibody
      • WARS protein antibody
      • WRS antibody
      see all

    Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] images

    • All lanes : Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : L6 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 53 kDa
    • ab109213, at 1/500 dilution, staining Tryptophanyl tRNA synthetase in paraffin-embedded Human breast tissue by Immunohistochemistry.

    References for Anti-Tryptophanyl tRNA synthetase antibody [EPR3423] (ab109213)

    ab109213 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"