Anti-TSC22 domain family, member 4 antibody (ab76915)

Overview

  • Product nameAnti-TSC22 domain family, member 4 antibody
    See all TSC22 domain family, member 4 primary antibodies
  • Description
    Mouse monoclonal to TSC22 domain family, member 4
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length human TSC22 domain family, member 4 protein (AAH01966) with a 26 kDa tag.

  • Positive control
    • Hela nuclear extract. Recombinant tagged full length TSC22 domain family, member 4 protein. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab76915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged TSC22 domain family, member 4 is approximately 0.3ng/ml when used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Anti-TSC22 domain family, member 4 antibody images

  • Anti-TSC22 domain family, member 4 antibody (ab76915) at 5 µg/ml + Recombinant tagged full length TSC22 domain family, member 4 protein at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 83 kDa (why is the actual band size different from the predicted?)
  • Anti-TSC22 domain family, member 4 antibody (ab76915) at 5 µg/ml + Hela nuclear extract at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 41 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • ab76915, at 10 µg/ml, staining TSC22 domain family, member 4 in Hela cells by Immunofluorescence
  • Detection limit for recombinant tagged TSC22 domain family, member 4 is approximately 0.3ng/ml as a capture antibody.

References for Anti-TSC22 domain family, member 4 antibody (ab76915)

ab76915 has not yet been referenced specifically in any publications.

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