Overview

  • Product nameAnti-TSEN2 antibody
    See all TSEN2 primary antibodies
  • Description
    Rabbit polyclonal to TSEN2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 395-444 (LRRPLSWKSL AALSRVSVNV SKELMLCYLI KPSTMTDKEM ESPECMKRIK) of Human TSEN2 (NP_079541).

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionConstitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
  • Tissue specificityIsoform 1 and isoform 2 are widely expressed at very low level.
  • Involvement in diseaseDefects in TSEN2 are the cause of pontocerebellar hypoplasia type 2B (PCH2B) [MIM:612389]. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings.
  • Sequence similaritiesBelongs to the tRNA-intron endonuclease family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Nucleus > nucleolus. May be transiently localized in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsSen2 antibody
    • MGC2776 antibody
    • MGC4440 antibody
    • SEN2 antibody
    • SEN2_HUMAN antibody
    • SEN2L antibody
    • tRNA intron endonuclease Sen2 antibody
    • tRNA splicing endonuclease 2 homolog (SEN2, S. cerevisiae) antibody
    • tRNA splicing endonuclease subunit Sen2 antibody
    • tRNA-intron endonuclease sen2 antibody
    • tRNA-splicing endonuclease subunit Sen2 antibody
    • TSEN 2 antibody
    • TSEN2 antibody
    see all

Anti-TSEN2 antibody images

  • Anti-TSEN2 antibody (ab125383) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG HRP

    Predicted band size : 53 kDa

References for Anti-TSEN2 antibody (ab125383)

ab125383 has not yet been referenced specifically in any publications.

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