Overview

  • Product name
    Anti-TSEN34 antibody
  • Description
    Mouse polyclonal to TSEN34
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human TSEN34 protein (AAH00944.1, 390 a.a)

  • Positive control
    • TSEN34 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
  • Involvement in disease
    Pontocerebellar hypoplasia 2C
  • Sequence similarities
    Belongs to the tRNA-intron endonuclease family.
  • Cellular localization
    Nucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsSen34 antibody
    • LENG 5 antibody
    • LENG5 antibody
    • Leukocyte receptor cluster (LRC) member 5 antibody
    • Leukocyte receptor cluster member 5 antibody
    • SEN 34 antibody
    • SEN34 antibody
    • SEN34_HUMAN antibody
    • SEN34L antibody
    • tRNA intron endonuclease Sen34 antibody
    • tRNA splicing endonuclease 34 homolog antibody
    • tRNA splicing endonuclease subunit 34 antibody
    • tRNA splicing endonuclease subunit Sen34 antibody
    • tRNA-intron endonuclease Sen34 antibody
    • tRNA-splicing endonuclease subunit Sen34 antibody
    • TSEN 34 antibody
    • Tsen34 antibody
    see all

Images

  • All lanes : Anti-TSEN34 antibody (ab68868) at 1/500 dilution

    Lane 1 : TSEN34 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References

ab68868 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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