Overview

  • Product nameAnti-TSEN54 antibody
    See all TSEN54 primary antibodies
  • Description
    Rabbit polyclonal to TSEN54
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human TSEN54.

  • Positive control
    • 3T3 and Jurkat cell lysate, Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab110723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionNon-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3' end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
  • Involvement in diseaseDefects in TSEN54 are the cause of pontocerebellar hypoplasia type 4 (PCH4) [MIM:225753]. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH4 is characterized by severe course and early lethality.
    Defects in TSEN54 are the cause of pontocerebellar hypoplasia type 2A (PCH2A) [MIM:277470]. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings.
  • Sequence similaritiesBelongs to the SEN54 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Nucleus > nucleolus. May be transiently localized in the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsSen54 antibody
    • PCH2A antibody
    • PCH4 antibody
    • sen54 antibody
    • SEN54 homolog antibody
    • SEN54_HUMAN antibody
    • SEN54L antibody
    • tRNA-intron endonuclease SEN54 antibody
    • tRNA-splicing endonuclease subunit SEN54 antibody
    • Tsen54 antibody
    • TSEN54 tRNA splicing endonuclease subunit antibody
    see all

Anti-TSEN54 antibody images

  • All lanes : Anti-TSEN54 antibody (ab110723) at 1/500 dilution

    Lane 1 : 3T3 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Jurkat cell lysate with blocking peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 59 kDa
  • Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using ab110723 at a dilution of 1/50. Right panel is also treated with blocking peptide.

References for Anti-TSEN54 antibody (ab110723)

ab110723 has not yet been referenced specifically in any publications.

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