Fusion protein containing amino acids 167-374 of the TSG101 protein expressed in E. coli.
Our Abpromise guarantee covers the use of ab83 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23045692|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/100 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).|
|IHC-P||1/100. Do not perform antigen retrieval.|
Immunohistochemical analysis of paraffin-embedded colon carcinoma tissue labeling TSG101 with ab83 at 1/100 dilution.
Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.
Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.