Anti-TSG101 antibody - Aminoterminal end (ab50582)


  • Product nameAnti-TSG101 antibody - Aminoterminal end
    See all TSG101 primary antibodies
  • Description
    Rabbit polyclonal to TSG101 - Aminoterminal end
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to N terminal amino acids 2-20 of Human TSG101

  • Positive control
    • Cell lysate of Rat-1 cells, NIH3T3 cells, A431 cells and rat brain extract (S1 cytosolic fraction)



Our Abpromise guarantee covers the use of ab50582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 44 kDa).


  • FunctionComponent of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
    Contains 1 SB (steadiness box) domain.
    Contains 1 UEV (ubiquitin E2 variant) domain.
  • DomainThe UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
    The coiled coil domain may interact with stathmin.
    The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
  • Post-translational
    Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
  • Cellular localizationCytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESCRT I complex subunit TSG101 antibody
    • ESCRT-I complex subunit TSG101 antibody
    • TS101_HUMAN antibody
    • TSG 10 antibody
    • TSG 101 antibody
    • TSG10 antibody
    • Tsg101 antibody
    • Tumor susceptibility gene 10 antibody
    • Tumor susceptibility gene 101 antibody
    • Tumor susceptibility gene 101 protein antibody
    • Tumor susceptibility protein antibody
    • Tumor susceptibility protein isoform 3 antibody
    • VPS 23 antibody
    • VPS23 antibody
    see all

Anti-TSG101 antibody - Aminoterminal end images

  • All lanes : Anti-TSG101 antibody - Aminoterminal end (ab50582) at 1 µg/ml

    Lane 1 : Rat1
    Lane 2 : NIH3T3
    Lane 3 : A431
    Lane 4 : A431 with Immunizing peptide at 10 µg/ml

    Lysates/proteins at 20 µg/ml per lane.

    Goat Anti-Rabbit IgG, Peroxidase conjugate at 1/2000 dilution
    developed using the ECL technique

    Predicted band size : 44 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TSG101 antibody - Aminoterminal end (ab50582)

ab50582 has not yet been referenced specifically in any publications.

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