Recombinant
RabMAb

Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Overview

  • Product name
    Anti-TSG101 antibody [EPR7130(B)]
    See all TSG101 primary antibodies
  • Description
    Rabbit monoclonal [EPR7130(B)] to TSG101
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TSG101 aa 1-100.

  • Positive control
    • WB: K562, Jurkat, and SH-SY5Y cell lysates. Human, mouse and rat brain tissue lysates. IHC-P: Human kidney tissue. ICC/IF: HEK293 cells. Flow Cyt: HeLa and K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-TSG101 antibody (Alexa Fluor® 488) [EPR7130(B)] (ab207663)

    Anti-TSG101 antibody (Alexa Fluor® 647) [EPR7130(B)] (ab207664)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab125011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Flow Cyt 1/40.

For unpurified use at 1/3.





ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

For unpurified use at 1/10.

IHC-P 1/500.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/30.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
    • Tissue specificity
      Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
    • Sequence similarities
      Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
      Contains 1 SB (steadiness box) domain.
      Contains 1 UEV (ubiquitin E2 variant) domain.
    • Domain
      The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
      The coiled coil domain may interact with stathmin.
      The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
    • Post-translational
      modifications
      Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
    • Cellular localization
      Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
    • Information by UniProt
    • Database links
    • Alternative names
      • ESCRT I complex subunit TSG101 antibody
      • ESCRT-I complex subunit TSG101 antibody
      • TS101_HUMAN antibody
      • TSG 10 antibody
      • TSG 101 antibody
      • TSG10 antibody
      • Tsg101 antibody
      • Tumor susceptibility 101 antibody
      • Tumor susceptibility gene 10 antibody
      • Tumor susceptibility gene 101 antibody
      • Tumor susceptibility gene 101 protein antibody
      • Tumor susceptibility protein antibody
      • Tumor susceptibility protein isoform 3 antibody
      • VPS 23 antibody
      • VPS23 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with unpurified ab125011 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/10000 dilution (purified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/500 dilution (unpurified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 7000 (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Mouse heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 44 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

      Lane 1 : Human Brain lysate
      Lane 2 : K562 lysate
      Lane 3 : Jurkat lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 44 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with purified ab125011 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells labelling TSG101 (red) with unpurified ab125011 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells labelling TSG101 (red) with purified ab125011 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Flow cytometry analysis of permeabilized HeLa cells labeling TSG101 with unpurified ab125011 at 1/3 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • Flow cytometry analysis of permeabilized HeLa cells labeling TSG101 with purified ab125011 at 1/40 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • Flow cytometric analysis of permeabilized K562 cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Green - Isotype control, rabbit monoclonal IgG.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Zheng T  et al. Plasma Exosomes Spread and Cluster Around ß-Amyloid Plaques in an Animal Model of Alzheimer's Disease. Front Aging Neurosci 9:12 (2017). WB . Read more (PubMed: 28203202) »
    • Saha S  et al. Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury. Nat Commun 7:13096 (2016). WB ; Mouse . Read more (PubMed: 27734833) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - other (Exosomes from HK-2 cells)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    50 µg
    Specification
    Exosomes from HK-2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Eric Barros

    Verified customer

    Submitted Oct 24 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast Cancer Cell Line MDA-MB231)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Breast Cancer Cell Line MDA-MB231
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Mr. Gabriel Ortega

    Verified customer

    Submitted Mar 06 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up