Recombinant Anti-TSG101 antibody [EPR7130(B)] (ab125011)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7130(B)] to TSG101
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TSG101 antibody [EPR7130(B)]
See all TSG101 primary antibodies -
Description
Rabbit monoclonal [EPR7130(B)] to TSG101 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: Mouse heart tissue lysates. K-562, Jurkat, SH-SY5Y, NIH/3T3 and PC-12 whole cell lysate. Mouse and rat heart and brain lysate. Flow Cyt: HeLa cells. ICC/IF: Jurkat cells and HeLa cells. IHC-P: Rat kidney tissue, mouse kidney tissue, human thyroid cancer tissue, human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 4.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7130(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)
- Alexa Fluor® 488 Anti-TSG101 antibody [EPR7130(B)] (ab207663)
- Alexa Fluor® 647 Anti-TSG101 antibody [EPR7130(B)] (ab207664)
- PE Anti-TSG101 antibody [EPR7130(B)] (ab209927)
- Alexa Fluor® 555 Anti-TSG101 antibody [EPR7130(B)] (ab214404)
- Anti-TSG101 antibody [EPR7130(B)] - Low endotoxin, Azide free (ab216447)
- APC Anti-TSG101 antibody [EPR7130(B)] (ab316299)
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Compatible Secondaries
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Isotype control
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Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Jurkat whole cell lysate (ab7899)
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (7) |
1/1000 - 1/10000. Detects a band of approximately 47, 52 kDa (predicted molecular weight: 44 kDa).
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Flow Cyt (Intra) |
1/20.
For unpurified use at 1/3. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (3) |
Use a concentration of 1 µg/ml.
For unpurified use at 1/10. This product gave a positive signal in HeLa fixed with 100% methanol (5 min). |
IHC-P |
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/30. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 47, 52 kDa (predicted molecular weight: 44 kDa). |
Flow Cyt (Intra)
1/20. For unpurified use at 1/3. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. For unpurified use at 1/10. This product gave a positive signal in HeLa fixed with 100% methanol (5 min). |
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30. |
Target
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Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. -
Tissue specificity
Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Contains 1 SB (steadiness box) domain.
Contains 1 UEV (ubiquitin E2 variant) domain. -
Domain
The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently. -
Post-translational
modificationsMonoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1. -
Cellular localization
Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis. - Information by UniProt
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Database links
- Entrez Gene: 7251 Human
- Entrez Gene: 22088 Mouse
- Entrez Gene: 292925 Rat
- Omim: 601387 Human
- SwissProt: Q99816 Human
- SwissProt: Q61187 Mouse
- SwissProt: Q6IRE4 Rat
- Unigene: 523512 Human
see all -
Alternative names
- ESCRT I complex subunit TSG101 antibody
- ESCRT-I complex subunit TSG101 antibody
- TS101_HUMAN antibody
see all
Images
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Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling TSG101 with Purified ab125011 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab125011 staining TSG101 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab125011 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TSG101 with Purified ab125011 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling TSG101 with Purified ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 6 : Mouse heart lysate
Lane 7 : Rat heart lysate
Lane 8 : Mouse brain lysate
Lane 9 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47, 52 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
The doublet are consistent with what have been described in the literature (PMID: 27882925, 31684046, 32932791, 11427703).
Image produced using the purified version.
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Different batches of ab125011 were tested on Mouse heart lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 45 kDa.
This data was generarted using the unpurified format.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (426)
ab125011 has been referenced in 426 publications.
- Ma L et al. Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair. Bioact Mater 19:626-641 (2023). PubMed: 35600968
- Wu Q et al. Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair. Bioact Mater 20:208-220 (2023). PubMed: 35702606
- Hirata T et al. N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells. iScience 26:105747 (2023). PubMed: 36590176
- He Y et al. Soluble PD-L1: a potential dynamic predictive biomarker for immunotherapy in patients with proficient mismatch repair colorectal cancer. J Transl Med 21:25 (2023). PubMed: 36639643
- Zhu C et al. Tumor-derived extracellular vesicles inhibit HGF/c-Met and EGF/EGFR pathways to accelerate the radiosensitivity of nasopharyngeal carcinoma cells via microRNA-142-5p delivery. Cell Death Discov 8:17 (2022). PubMed: 35013115