Overview

  • Product name
    Anti-TSGA14 antibody
  • Description
    Rabbit polyclonal to TSGA14
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    A region between residue 150 and 200 of human TSGA14 (NP_061188.1).

  • Positive control
    • HeLa and 293T whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified using an epitope specific to TSGA14 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Required during ciliogenesis for tubulin glutamylation in cilium. Probably acts by participating to the transport of TTLL6, a tubulin polyglutamylase, between the basal body and the cilium.
  • Tissue specificity
    Isoform 1 and isoform 4 are expressed in testis and fetal tissues.
  • Involvement in disease
    Defects in CEP41 are the cause of Joubert syndrome type 15 (JBTS15) [MIM:614464]. JBTS15 is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy, renal disease, liver fibrosis and polydactyly.
    Note=Genetic variations in CEP41 may be associated with susceptibility to autism.
  • Sequence similarities
    Belongs to the CEP41 family.
    Contains 1 rhodanese domain.
  • Domain
    Although it contains a rhodanese domain, does not display phosphatase activity, suggesting that the protein is enzymatically inactive (PubMed:22246503).
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Localizes mainly to the cilium basal body and in primary cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomal protein 41 kDa antibody
    • Centrosomal protein of 41 kDa antibody
    • CEP 41 antibody
    • Cep41 antibody
    • CEP41_HUMAN antibody
    • testis specific 14 antibody
    • testis specific gene A14 antibody
    • Testis specific gene A14 protein antibody
    • testis specific protein A14 antibody
    • Testis-specific gene A14 protein antibody
    see all

Images

  • All lanes : Anti-TSGA14 antibody (ab86180) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg/ml

    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 100 kDa,200 kDa,250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human TSGA14 by Immunoprecipitation, using ab86180 at 3µg/mg lysate. Image shows immunoprecipitated TSGA14 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab86180 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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