Anti-TSH alpha antibody [ME.132 ] (ab9510)

Overview

  • Product nameAnti-TSH alpha antibody [ME.132 ]See all TSH alpha primary antibodies ...
  • Description
    Mouse monoclonal [ME.132 ] to TSH alpha
  • SpecificityThis antibody shows cross reactivity with: hCG-beta = <1% hCG-alpha = <1% hLH = <1% hFSH = <1% hTSH = <100% Affinity = 4 x 1010
  • Tested applicationsELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    hTSH-alpha, MW 13.5 kDa

  • Positive control
    • IHC-P: FFPE human breast tissue sections.
  • General notesP.I. 6.0-7.0

Properties

Applications

Our Abpromise guarantee covers the use of ab9510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceThyroid stimulating hormone (TSH), also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • aGSU antibody
    • alpha GSU antibody
    • AlphaSU antibody
    • Chorionic gonadotropin alpha polypeptide antibody
    • Glycoprotein hormones alpha chain precursor antibody
    • Glycoprotein hormones alpha polypeptide antibody
    • Glycoprotein hormones alpha polypeptide antibody
    • GPHalpha antibody
    • Thyroid Stimulating Hormone antibody
    • Thyroid Stimulating Hormone antibody
    • Thyroid Stimulation Hormone antibody
    • Thyroid Stimulation Hormone antibody
    • Thyrotropin alpha subunit antibody
    see all

Anti-TSH alpha antibody [ME.132 ] images

  • IHC image of TSH alpha staining in human breast formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9510, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TSH alpha antibody [ME.132 ] (ab9510)

ab9510 has not yet been referenced specifically in any publications.

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We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the stor...

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