Overview

  • Product name
    Anti-TSH antibody, prediluted
    See all TSH primary antibodies
  • Description
    Rabbit polyclonal to TSH, prediluted
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human TSH

  • Positive control
    • Human anterior pituitary

Properties

Applications

Our Abpromise guarantee covers the use of ab64486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Ready to use for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • CG ALPHA antibody
      • CGA antibody
      • Chorionic gonadotropin alpha polypeptide antibody
      • FSHA antibody
      • Glycoprotein hormones alpha polypeptide antibody
      • GPHA1 antibody
      • Thyroid stimulating hormone beta subunit antibody
      • Thyrotropin beta subunit antibody
      • TSH beta antibody
      • TSHA antibody
      • TSHB antibody
      see all

    Images

    • ab64486 undiluted staining human anterior pituitary; formalin fixed, paraffin embedded.

    References

    This product has been referenced in:
    • Mutinati M  et al. Localization of thyrotropin receptor and thyroglobulin in the bovine corpus luteum. Anim Reprod Sci 118:1-6 (2010). IHC-P ; Cow . Read more (PubMed: 19553036) »

    See 1 Publication for this product

    Customer reviews and Q&As

    I am glad to hear that this antibody worked well in IHC with zebrafish samples, as we have not previously tested it with zebrafish and did not know that it cross-reacts. We have also not tested this antibody in Western blot so we don't a fully optimize...

    Read More

    Thank you for contacting Abcam.

    The concentration of ab64486, lot#*is 1 ug/ml.

    Please do not hesitate to contact me with additional questions or concerns.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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