Recombinant
RabMAb

Anti-TSH beta antibody [EPR8198] (ab155958)

Overview

  • Product name
    Anti-TSH beta antibody [EPR8198]
    See all TSH beta primary antibodies
  • Description
    Rabbit monoclonal [EPR8198] to TSH beta
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human TSH beta (UniProt: P01222).

  • Positive control
    • Rabbit pituitary tissue lysate; Human recombinant TSH alpha/beta protein; Human pituitary tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab155958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance
      Various hormones are secreted from the anterior pituitary during development and growth. Thyroid Stimulating Hormone (TSH) is secreted from the anterior pituitary and controls thyroid structure and metabolism
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • CHNG4 antibody
      • Thyroid stimulating hormone beta subunit antibody
      • Thyroid stimulating hormone, beta precursor antibody
      • Thyrotropin beta subunit antibody
      • TSHB antibody
      see all

    Images

    • Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution + Rabbit pituitary tissue lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 16 kDa
    • Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution + Human recombinant TSH alpha/beta protein at 0.01 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 16 kDa
    • Immunohistochemical analysis of paraffin-embedded Human pituitary tissue, labeling TSH beta with ab155958 at 1/250 dilution.

    References

    ab155958 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Purified protein (pituitary)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    0.7 µg
    Specification
    pituitary
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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